Laboratory of Physical Biochemistry | Intercollegiate Faculty of Biotechnology UG&MUG

Laboratory of Physical Biochemistry

Last modified: 
2018, May 18 - 12:32pm


Research areas:

  • Functions of MTHFR and CBS genes during formation of homocysteine in the context of therapeutic and preventive aspects in patients with ischemic brain stroke
  • Effects of using silicone implants by determining the influence of linear and cyclic polysiloxanes on stability and structure of proteins
  • Practical applications of chromatographic methods of quality control and analytical method validation
  • Methods of isolation and testing of new compounds with potential pharmaceutical application
  • Biophysical analysis of protein structure and function


Laboratory of Physical Biochemistry

Department of Molecular and Cellular Biology
Intercollegiate Faculty of Biotechnology UG&MUG
Abrahama 58 Street, 80-307 Gdańsk, Poland

Office: Stanisława Urbańska
Tel.:+48 58 523 6421

team prof. Banecki


Head: Prof. Bogdan Banecki [link]
Tel.:+48 58 523 6324 [e-mail]

List of Employees and PhD students:

dr Danuta Gutowska-Owsiak [link]
tel.:+48 58 523 6333 [e-mail]
dr Leszek Kadziński [link]
tel.:+48 58 523 6429 [e-mail]

MSc Grzegorz Gawron
tel.:+48 58 523 6429 [e-mail]
MSc Paulina Tomiczek
tel.:+48 58 523 6429 [e-mail]
MSc Alicja Filipek
tel.:+48 58 523 6429 [e-mail]


  1. Mozolewski, Pawel, Jakobkiewicz-Banecka Joanna, Wegrzyn Grzegorz,  Banecki, Bogdan, Gabig-Ciminska Magdalena. Non-steroidal anti-inflammatory drugs are safe with respect to the transcriptome of human dermal fibroblasts. European Journal of Pharmacology 2018, 818: 206-210 (doi: 10.1016/j.ejphar.2017.10.040

  2. Smolińska Elwira, Moskot Marta, Jakóbkiewicz-Banecka Joanna, Węgrzyn Grzegorz, Banecki Bogdan, Szczerkowska-Dobosz Aneta, Purzycka-Bohdan Dorota, Gabig-Cimińska Magdalena. Molecular action of isoflavone genistein in the human epithelial cell line HaCaT. PLoS ONE 2018, (w druku) (doi: 10.1371/journal.pone.0192297)

  3. Gutowska-Owsiak Danuta, Bernardino de La Serna Jorge, Fritzsche Marco, Naeem Aishath, Podobas Ewa I., Leeming Michael, Colin-York Huw, O’Shaughnessy Ryan, Eggeling Christian, Ogg Graham S. Orchestrated control of filaggrin–actin scaffolds underpins cornification. Cell Death & Disease 2018, 9: 412 (doi: 10.1038/s41419-018-0407-2)

  4. Moskot Marta, Bocheńska Katarzyna, Jakóbkiewicz-Banecka Joanna, Banecki Bogdan, Gabig-Ciminska Magdalena. Abnormal Sphingolipid World in Inflammation Specific for Lysosomal Storage Diseases and Skin Disorders. International Journal of Molecular Sciences 2018, 19: 247 (doi: 10.3390/ijms19010247)

  5. Mozolewski Paweł, Moskot Marta, Jakóbkiewicz-Banecka Joanna, Węgrzyn Grzegorz, Bocheńska Katarzyna, Banecki Bogdan, Gabig-Cimińska Magdalena. Nonsteroidal anti-inflammatory drugs modulate cellular glycosaminoglycan synthesis by affecting EGFR and PI3K signaling pathways. Scientific Reports 2018, 7: 43154 (doi: 10.1038/srep43154)

  6. Śledz Wojciech, Żołędowska Sabina, Motyka AgataKadziński Leszek, Banecki Bogdan. Growth of bacterial phytopathogens in animal manures. Acta Biochimica Polonica 2017, 64(1): 151-159 (doi: 10.18388/abp.2016_1389)

  7. Jakobkiewicz-Banecka Joanna, Gabig-Ciminska Magdalena, Kloska Anna, Malinowska Marcelina, Piotrowska Ewa, Banecka-Majkutewicz Zyt, Banecki Bogdan, Wegrzyn  Alicja, Wegrzyn Grzegorz. Glycosaminoglycans and mucopolysaccharidosis type III. Frontiers in Bioscience-Landmark. 2016, 211393-1409 (doi: 10.2741/4463).

  8. Grabowski MichałBanecki BogdanKadziński Leszek, Jakobkiewicz-Banecka JoannaGabig-Ciminska  Magdalena, Wegrzyn Alicja, Wegrzyn Grzegorz, Banecka-Majkutewicz, Zyta. The model homologue of the partially defective human 5,10-methylenetetrahydrofolate reductase, considered as a risk factor for stroke due to increased homocysteine level, can be protected and reactivated by heat shock proteins. Metabolic Brain Disease 2016 31(5):1041-1045 (DOI: 10.1007/s11011-016-9844-8)

  9. Glaza Przemysław, Osipiuk Jerzy, Wenta Tomasz, Zurawa-Janicka Dorota, Jarząb Mirosław, Lesner Adam, Banecki Bogdan, Skórko-Glonek Joanna, Joachimiak Andrzej, Lipińska Barbara. Structural and functional analysis of human HtrA3 protease and its subdomains. PLoS ONE 2015, 10(6): art. no e0131142 (1-24) (doi: 10.1371/journal.pone.0131142).

  10. Grabowski MichałBanecki BogdanKadziński Leszek, Jakóbkiewicz-Banecka Joanna, Kaźmierkiewicz Rajmund, Gabig-Cimińska Magdalena, Węgrzyn Grzegorz, Węgrzyn Alicja, Banecka-Majkutewicz Zyta. Genistein inhibits activities of methylenetetrahydrofolate reductase and lactate dehydrogenase, enzymes which use NADH as a substrate. Biochemical and Biophysical Research Communications 2015, 465(3): 363-367 (doi: 10.1016/j.bbrc.2015.08.004).

  11. Kadziński Leszek, Prokopowicz Magdalena, Jakóbkiewicz-Banecka Joanna, Gabig-Cimińska Magdalena, Łukasiak Jerzy, Banecki Bogdan. Effect of Silicone on the Collagen Fibrillogenesis and Stability. Journal of Pharmaceutical Sciences 2015, 104(4): 1275-1281 (doi: 10.1002/jps.24351).

  12. Koper Tomasz, Polit Agnieszka, Sobiecka-Szkatula Anna, Węgrzyn Katarzyna, Scire Andrea, Figaj Donata, Kadziński Leszek, Zarzecka Urszula, Żurawa-Janicka Dorota, Banecki Bogdan, Lesner Adam, Tanfani Fabio, Lipińska Barbara, Skórko-Glonek Joanna. Analysis of the Link between the Redox State and Enzymatic Activity of the HtrA (DegP) Protein from Escherichia coliPLoS ONE 2015, art. no e0117413 (1-24) (doi: 10.1371/journal.pone.0117413).

  13. Moskot Marta, Jakóbkiewicz-Banecka Joanna, Kloska Anna, Smolińska Elwira, Mozolewski Paweł, Malinowska Marcelina, Rychłowski Michał, Banecki Bogdan, Węgrzyn Grzegorz, Gabig-Cimińska Magdalena. Modulation of expression of genes involved in glycosaminoglycan metabolism and lysosome biogenesis by flavonoids. Scientific Reports 2015, 5: art. no 9378 (1-13) (doi: 10.1038/srep09378).

  14. Moskot Marta, Jakóbkiewicz-Banecka Joanna, Smolińska Elwira, Banecki Bogdan, Węgrzyn Grzegorz, Gabig-Cimińska Magdalena. Activities of genes controlling sphingolipid metabolism in human fibroblasts treated with flavonoids. Metabolic Brain Disease 2015, 30(5): 1257-1267 (doi: 10.1007/s11011-015-9705-x).

  15. Banecka-Majkutewicz Zyta, Grabowski MichałKadziński LeszekPapkov Aliaksei, Węgrzyn Alicja, Banecki Bogdan. Increased levels of antibodies against heat shock proteins in stroke patients. Acta Biochimica Polonica2014, 61(2): 379-383 (pdf).
  16. Figaj Donata, Giełdon Artur, Polit Agnieszka, Sobiecka-Szkatuła Anna, Koper Tomasz, Denkiewicz Milena, Banecki Bogdan, Lesner Adam, Ciarkowski Jerzy, Lipińska Barbara, Skórko-Glonek Joanna. The la loop as an important regulatory element of the HtrA (DegP) protease from Escherichia coli structural and functional studies. Journal of Biological Chemistry 2014, 289(22): 15880-15893 (doi: 10.1074/jbc.M113.532895).

  17. Jakóbkiewicz-Banecka Joanna, Gabig-Cimińska Magdalena, Banecka-Majkutewicz Zyta, Banecki Bogdan, Węgrzyn Alicja, Węgrzyn Grzegorz. Factors and processes modulating phenotypes in neuronopathic lysosomal storage diseases. Metabolic Brain Disease 2014, 29(1): 1-8 (doi: 10.1007/s11011-013-9455-6).

  18. Siwińska Joanna, Kadziński Leszek, Banasiuk Rafał, Gwizdek-Wiśniewska Anna, Olry Alexandre, Banecki Bogdan, Łojkowska Ewa, Ihnatowicz Anna. Identification of QTL affecting scopolin and scopoletin biosynthesis in Arabidopsis thalianaBMC Plant Biology 2014przyjęta do druku (doi: 10.1186/s12870-014-0280-9).

  19. Banecka-Majkutewicz Zyta, Sawuła WojciechKadziński Leszek, Węgrzyn Alicja, Banecki Bogdan. Homocysteine, heat shock proteins, genistein and vitamins in ischemic stroke - pathogenic and therapeutic implications. Acta Biochimica Polonica 2012, 59(4): 495-499.

  20. Grabowski MichałBanasiuk Rafał, Węgrzyn Alicja, Kędzierska Barbara, Lica Jan, Banecka-Majkutewicz Zyta, Banecki Bogdan. Role of heat-shock proteins and cobalamine in maintaining methionine synthase activity. Acta Biochimica Polonica 2012, 59(4): 489-493.

  21. Sznarkowska A.Maleńczyk K.Kadziński L.Bielawski K. P.Banecki B.Zawacka-Pankau J. Targeting of p53 and its homolog p73 by protoporphyrin IX. FEBS Letters 2011, 585(1): 255-260.

  22. Ferens B.Kawiak A.Banecki B.Bielawski K. P.Zawacka-Pankau J. Aberration of the enzymatic activity of Fhit tumor suppressor protein enhances cancer cell death upon photodynamic therapy similarly to that driven by wild-type Fhit. Cancer Letters 2009, 280(1): 101-109.

  23. Mrozek J., Lewandowska A., Guzow K., Malicka J., Banecki B., Wiczk W. Influence of Dab2 and Pro3 configuration of [Leu]-enkephalins on the interactions with β-cyclodextrin studied by fluorescence spectroscopy, microcalorimetry and 1H NMR spectroscopy. Journal of Inclusion Phenomena and Macrocyclic Chemistry 2009, 65(3-4): 361-375.

  24. Sawuła W., Banecka-Majkutewicz Z., Kadziński L., Jakóbkiewicz-Banecka J., Węgrzyn G., Nyka W., Banecki B.Homocysteine level and metabolism in ischemic stroke in the population of Northern Poland. Clinical Biochemistry 2009, 42(6): 442-447.