Laboratory of Molecular Bacteriology

Research areas:

• Molecular biology of Bacillus subtilis
• Protein phosphorylation in Bacillus subtilis
• Bacterial spore properties
• Oral vaccine based on spores
• Spore as carrier for enzymes

Laboratory of Molecular Bacteriology

Intercollegiate Faculty of Biotechnology UG&MUG
Dębinki 1 Street, 80-211 Gdańsk, Poland

tel.: +48 58 3491435 

STAFF LIST

Head: prof. Michał Obuchowski [link]
tel.: +48 58 3491438 [e-mail]

List of Employees and PhD students:

Dsc. Krzysztof Hinc [link]
Tel.: +48 58 349 1412 [e-mail] 
Dsc. Adam Iwanicki [link]
Tel.: +48 58 349 1411 [e-mail]
PhD Alessandro Negri

MSc Tomasz Łęga
MSc Roberta Gatta [email]
MSc Katarzyna Pruska [email]

1. Gatta Roberta, Wiese Andrzej, Iwanicki Adam, Obuchowski Michał. Influence of glucose on swarming and quorum sensing of Dickeya solani. PLoS ONE 2022, 17(2): e0263124 (doi: 10.1371/journal.pone.0263124)

2. Radzki Dominik, Wilhelm-Węglarz Marta, Pruska Katarzyna, Kusiak Aida, Ordyniec-Kwaśnica Iwona. A fresh look at mouthwashes - what is inside and what is it for?. International Journal of Environmental Research and Public Health 2022, 19(7): 3926 (doi: 10.3390/ijerph19073926)

3. Krzyżanowska Dorota M., Iwanicki Adam, Czajkowski Robert, Jafra Sylwia. High-quality complete genome resource of tomato rhizosphere strain Pseudomonas donghuensis P482, a representative of a species with biocontrol activity against plant pathogens. Molecular Plant-Microbe Interactions 2021, (w druku) (doi: 10.1094/MPMI-06-21-0136-A)

4. Guzow Katarzyna, Mulkiewicz Ewa, Obuchowski Michał, Wiczk Wiesław. Biological activity of 3-(2-benzoxazol-5-yl)alanine derivatives. Amino Acids 2021, 53(8): 1257-1268 (doi: 10.1007/s00726-021-03030-7)

5. Hinc Krzysztof, Kabała Monika, Iwanicki Adam, Martirosian Gajane, Negri Alessandro, Obuchowski Michał. Complete genome sequence of the newly discovered temperate Clostridioides difficile bacteriophage phiCDKH01 of the family Siphoviridae. Archives of Virology 2021, 166(8): 2305-2310 (doi: 10.1007/s00705-021-05092-0)

6. Kosikowska-Adamus Paulina, Sikorska Emilia, Wyrzykowski Dariusz, Walewska Aleksandra, Golda Anna, Deptuła Milena, Obuchowski Michał, Prahl Adam, Pikuła Michał, Lesner Adam. Lipidation of temporin-1CEb derivatives as a tool for activity improvement, pros and cons of the approach. International Journal of Molecular Sciences 2021, 22(13): 6679 (doi: 10.3390/ijms22136679)

7. Gonciarz Weronika, Krupa Agnieszka, Hinc Krzysztof, Obuchowski Michał, Moran Anthony P., Gajewski Adrian, Chmiela Magdalena. The effect of Helicobacter pylori infection and different H. pylori components on the proliferation and apoptosis of gastric epithelial cells and fibroblasts. PLoS ONE 2019, 14(8): e0220636 (doi: 10.1371/journal.pone.0220636)

8. Gonciarz Weronika, Walencka Maria, Moran Anthony P., Hinc Krzysztof, Obuchowski Michał, Chmiela Magdalena. Upregulation of MUC5AC production and deposition of LEWIS determinants by HELICOBACTER PYLORI facilitate gastric tissue colonization and the maintenance of infection. Journal of Biomedical Science 2019, 26: 23 (doi: 10.1186/s12929-019-0515-z)

9. Grela Anna, Jamrożek Inga, Hubisz Marta, Iwanicki Adam, Hinc Krzysztof, Kaźmierkiewicz Rajmund, Obuchowski Michał. Positions 299 and 302 of the GerAA subunit are important for function of the GerA spore germination receptor in Bacillus subtilis. PLOS One 2018, 13(6); e0198561 (doi: 10.1371/journal.pone.0198561)

10. Zyśk Marlena, Gapys Beata, Ronowska Anna, Gul-Hinc Sylwia, Erlandsson Anna, Iwanicki Adam, Sakowicz-Burkiewicz Monika, Szutowicz Andrzej, Bielarczyk Hanna.. Protective effects of voltage-gated calcium channel antagonists against zinc toxicity in SN56 neuroblastoma cholinergic cells . PLoS ONE 2018, 13(12): e0209363 (doi: 10.1371/journal.pone.0209363)

11. Potocki Wojciech, Negri Alessandro, Peszyńska-Sularz Grażyna, Hinc Krzysztof, Obuchowski Michał, Iwanicki Adam. IL-1 fragment modulates immune response elicited by recombinant Bacillus subtilis spores presenting an antigen/adjuvant chimeric protein. Molecular Biotechnology 2018, 60(11): 810-819 (doi: 10.1007/s12033-018-0117-0)

12. Piekarska Aurelia, Pełka Paulina, Peszynska-Sularz Grażyna, Negri Alessandro, Hinc Krzysztof, Obuchowski Michał, Iwanicki Adam. The choice of the anchoring protein influences the interaction of recombinant Bacillus spores with the immune system. Acta Biochimica Polonica 2017, 64(2): 239-244 (doi: 10.18388/abp.2016_1315)

13. Rostami Amin, Hinc Krzysztof , Goshadrou Fatemeh, Shali Abbas,  Bayat Mandieh, Hassanzadeh Malihe, Amanlou Massoud, Eslahi Negin, Ahmadian Gholamreza. Display of B-pumilus chitinase on the surface of B-subtilis spore as a potential biopesticide. Pesticide Biochemistry and Physiology 2017, 140: 17-23 (doi: 10.1016/j.pestbp.2017.05.008)     

14. Potocki Wojciech, Negri Alessandro, Peszyńska‑Sularz Grażyna, Hinc Krzysztof, Obuchowski Michał, Iwanicki Adam. The combination of recombinant and non-recombinant Bacillus subtilis spore display technology for presentation of antigen and adjuvant on single spore. Microbial Cell Factories 2017, 16: 151 (doi: 10.1186/s12934-017-0765-y)

15. Łęga Tomasz, Weiher Paulina, Obuchowski Michał, Nidzworski Dawid. Presenting Influenza A M2e Antigen on Recombinant Spores of Bacillus subtilis. PLOS ONE 2016, 11(11): e0167225 (doi: 10.1371/journal.pone.0167225)

16. Mnich Eliza, Kowalewicz-Kulbat Magdalena, Sicinska, Paulina, Hinc Krzysztof, Obuchowski Michał, Gajewski  Adrian, Moran  Anthony P., Chmiela Magdalena. Impact of Helicobacter pylori on the healing process of the gastric barrier. World Journal of Gastroenterology 2016, 22(33):  7536-7558 (doi: 10.3748/wjg.v22.i33.7536)

17. Krzyżanowska Dorota M, Ossowicki Adam, Rajewska Magdalena, Maciag Tomasz, Jabłonska  Magdalena, Obuchowski  Michal, Heeb Stephan, Jafra  Sylwia.  When Genome-Based Approach Meets the "Old but Good": Revealing Genes Involved in the Antibacterial Activity of Pseudomonas sp P482 against Soft Rot Pathogens. Frontiers in Microbiology 2016 , 7: 782 (doi: 10.3389/fmicb.2076.00782)

18. Gajewski Adrian, Mnich Eliza, Szymański Karol, Hinc Krzysztof, Obuchowski Michał, Moran Anthony P., Chmiela Magdalena. Helicobacter pylori antigens, acetylsalicylic acid, LDL and 7-ketocholesterol - their potential role in destabilizing the gastric epithelial cell barrier. An in vitro model of Kato III cells. Acta Biochimica Polonica 2016, 63(1): 145-152 (doi: 10.18388/abp.2015_1122).

19. Mnich Eliza, Gajewski Adrian, Rudnicka Karolina, Gonciarz Weronika, Stawerski Paweł, Hinc Krzysztof, Obuchowski Michał, Chmiela Magdalena. Immunoregulation of antigen presenting and secretory functions of monocytic cells by Helicobacter pylori antigens in relation to impairment of lymphocyte expansion. Acta Biochimica Polonica 2015, 62(4): 641-650 (doi:10.18388/abp.2015_1045)

20. Kosikowska Paulina, Pikula Michał, Langa Paulina, Trzonkowski Piotr, Obuchowski Michał, Lesner Adam. Synthesis and Evaluation of Biological Activity of Antimicrobial – Pro-Proliferative Peptide Conjugates. PLoS ONE 2015, 10(10): art. no e0140377 (1-16) (doi: 10.1371/journal.pone.0140377).

21. Stasiłojć Małgorzata, Hinc Krzysztof, Peszyńska-sularz Grażyna, Obuchowski Michał, Iwanicki Adam. Recombinant Bacillus subtilis spores elicit Th1/Th17-polarized immune response in a murine model of Helicobacter pylori vaccination. Molecular Biotechnology 2015, 57(8): 685-691 (doi: 10.1007/s12033-015-9859-0).

22. Hinc Krzysztof, Stasiłojć Małgorzata, Piątek Iwona, Peszyńska-Sularz Grażyna, Isticato Rachele, Ricca Ezio, Obuchowski Michał, Iwanicki Adam. Mucosal adjuvant activity of IL-2 presenting spores of Bacillus subtilis in a murine model of Helicobacter pylori vaccination. PLoS ONE 2014, 9(4): art. no e95187 (1-9) (doi: 10.1371/journal.pone.0095187).

23. Iwanicki Adam, Piątek Iwona, Stasiłojć Małgorzata, Grela Anna, Łęga Tomasz, Obuchowski Michał, Hinc Krzysztof. A system of vectors for Bacillus subtilis spore surface display. Microbial Cell Factories 2014, 13: art. no 30: (1-9) (doi: 10.1186/1475-2859-13-30).

24. Golec P., Karczewska-Golec J., Voigt B., Albrecht D., Schweder T., Hecker M., Węgrzyn G., Łoś M. Proteomic profiles and kinetics of development of bacteriophage T4 and its rI and rIII mutants in slowly growing Escherichia coli. Journal of General Virology 2013, 94(Pt 4): 896-905.

25. Hinc Krzysztof, Iwanicki Adam, Obuchowski Michał. New stable anchor protein and peptide linker suitable for successful spore surface display in B. subtilis. Microbial Cell Factories 2013, 12: art. no 22 (1-8).

26. Iwanicki Adam, Hinc Krzysztof, Ronowicz Anna, Piotrowski Arkadiusz, Wołoszyk Aleksandra, Obuchowski Michał. A genome-wide transcriptional profiling of sporulating Bacillus subtilis strain lacking PrpE protein phosphatase. Molecular Genetics and Genomics 2013, 288(10): 469-481.

27. Krzyżanowska Dorota M., Iwanicki Adam, Ossowicki Adam, Obuchowski Michał, Jafra Sylwia. Genome sequence of Bacillus subtilis MB73/2, a soil isolate inhibiting the growth of plant pathogens Dickeya spp. and Rhizoctonia solani. Genome Announcements 2013, 1(3): art. no. e00238-13 (1-2).

28. Negri Alessandro, Potocki Wojciech, Iwanicki Adam, Obuchowski Michał, Hinc Krzysztof. Expression and display of Clostridium difficile protein FliD on the surface of Bacillus subtilis spores. Journal of Medical Microbiology 2013, 62(Pt 9): 1379-1385.

29. Sączewski Jarosław, Hinc Krzysztof, Obuchowski Michał, Gdaniec Maria. The tandem mannich-electrophilic amination reaction: a versatile platform for fluorescent probing and labeling. Chemistry-A European Journal 2013, 19(35): 11531-11535.

30. Tavassoli S., Hinc K., Iwanicki A., Obuchowski M., Ahmadian G. Investigation of spore coat display of Bacillus subtilis β-galactosidase for developing of whole cell biocatalyst. Archives of Microbiology 2013, 195(3): 197-202.

31. Golec Piotr, Karczewska-Golec Joanna, Łoś Marcin, Węgrzyn Grzegorz. Novel ZnO-binding peptides obtained by the screening of a phage display peptide library. Journal of Nanoparticle Research 2012, 14(11): art. no. 1218 (1-6).

32. Krzyżanowska Dorota, Obuchowski Michał, Bikowski Mariusz, Rychłowski Michał, Jafra Sylwia. Colonization of potato rhizosphere by GFP-tagged Bacillus subtilis MB73/2, Pseudomonas sp. P482 and Ochrobactrum sp. A44 shown on large sections of root using enrichment sample preparation and confocal laser scanning microscopy. Sensors 2012, 12(12): 17608-17619.

33. Gruszczyński P., Smalara K., Obuchowski M., Kaźmierkiewicz R. ATP and its N6-substituted analogues: parameterization, molecular dynamics simulation and conformational analysis. Journal of Molecular Modeling 2011, 17(5): 1081-1090.

34. Obuchowski Michał, Hinc Krzysztof. Przetrwalniki Bacillus subtilis - nośniki jadalnych szczepionek. Laboratorium - Przegląd Ogólnopolski 2011, 7-8: 39.

35. Gruszczyński Paweł, Obuchowski Michał, Kaźmierkiewicz Rajmund. Phosphorylation and ATP-binding induced conformational changes in the PrkC, Ser/Thr kinase from B. subtilis. Journal of Computer-Aided Molecular Design 2010, 24(9): 733-747.

36. Hinc Krzysztof, Ghandili Soheila, Karbalaee Gholamreza, Shali Abbas, Akbari Noghabi Kambiz, Ricca Ezio, Ahmadian Gholamreza. Efficient binding of nickel ions to recombinant Bacillus subtilis spores. Research in Microbiology 2010, 161(9): 757-764.

37. Hinc Krzysztof, Isticato Rachele, Dembek Marcin, Karczewska Joanna, Iwanicki Adam, Peszyńska-Sularz Grażyna, De Felice Maurilio, Obuchowski Michał, Ricca Ezio. Expression and display of UreA of Helicobacter acinonychis on the surface of Bacillus subtilis spores. Microbial Cell Factories 2010, 9: art. 2 (1-11).

38. Macur Katarzyna, Bączek Tomasz, Kaliszan Roman, Temporini Caterina, Corana Federica, Massolini Gabriella, Grzenkowicz-Wydra Jolanta, Obuchowski Michał. Correctness of protein identifications of Bacillus subtilis proteome with the indication on potential false positive peptides supported by predictions of their retention times. Journal of Biomedicine and Biotechnology 2010, art. 718142: 1-13.

39. Macur Katarzyna, Temporini Caterina, Massolini Gabriella, Grzenkowicz-Wydra Jolanta, Obuchowski Michał, Bączek Tomasz. Proteomic analysis of small acid soluble proteins in the spore core of Bacillus subtilis ΔprpE and 168 strains with predictions of peptides liquid chromatography retention times as an additional tool in protein identification. Proteome Science 2010, 8 (art. 60): 1-8.

40. Nagórska K., Ostrowski A., Hinc K., Holland I.B., Obuchowski M. Importance of eps genes from Bacillus subtilis in biofilm formation and swarming. Journal of Applied Genetics 2010, 51(3): 369-381.

41. Absalon C., Obuchowski M., Madec E., Delattre D., Holland I.B., Seror S.J. CpgA, EF-Tu and the stressosome protein YezB are substrates of the Ser/Thr kinase/phosphatase couple, PrkC/PrpC, in Bacillus subtilis. Microbiology-SGM 2009, 155(3): 932-943.

42. Hamze K., Julkowska D., Autret S., Hinc K., Nagórska K., Sekowska A., Holland I.B., Seror S.J. Identification of genes required for different stages of dendritic swarming in Bacillus subtilis, with a novel role for phrC. Microbiology-SGM 2009, 155(2): 398-412.