Laboratory of Experimental and Translational Immunology

PROFILE

We aim to understand immunological mechanisms which may influence response to antigen in the skin, with particular interest in atopic dermatitis. Given the importance of the epidermal barrier in the disease we also try to understand its role in the pathogenesis and response to allergens and pathogens. While we focus predominantly on the skin disease as a model body barrier dysfunction, we also investigate communication and antigen presentation in other conditions where immunological mechanism are dysregulated.

Scientific interests:

•  communication between cells during immune responses via secreted extracellular vesicles
•  epidermal barrier in health and disease
•  mechanisms of allergic inflammation
•  pathways of antigen presentation
• novel immunotherapy approaches

Laboratory website: www.dgo.ug.edu.pl

RESEARCH TEAM

Laboratory of Experimental and Translational Immunology

Intercollegiate Faculty of Biotechnology UG&MUG
Abrahama 58 Street, 80-307 Gdańsk, Poland

Head: Danuta Gutowska-Owsiak, MD, PhD, DSc 
Professor at the University of Gdańsk [link]

tel.: +48 58 523 6433 [e-mail]

List of Employees and PhD students:

PhD Chen Zhi Jane [link]
tel: +48 58 523 6408 [e-mail]
PhD Anna Biernacka [link]
tel.: +48 58 523 6319 [e-mail]
PhD Agnieszka Jabłońska [link]
tel.: +48 58 523 6319 [e-mail]
PhD Ewa Czechowska [link]
tel.: +48 58 523 6319

MSc Adrian Kobiela
tel.: +48 58 523 6319 [email]
MSc Paulina Jurkowska
tel.: +48 58 523 6319 [email]
MSc Dominika Miroszewska
tel.: +48 58 523 6319 [email]
MSc Victor Urbiola Salvador
tel.: +48 58 523 6319 [email]
MSc Kinga Panek
tel.: +48 58 523 6319 [email]
MSc Argho Aninda Paul
tel.: +48 58 523 6319 [email]
MSc Mikołaj Klimczuk
tel.: +48 58 523 6319 [email]

PROJECTS

PUBLICATIONS

1. Kobiela Adrian, Hovhannisyan Lilit, Jurkowska Paulina, Bernardino dela Serna Jorge, Bogucka Aleksandra, Deptuła Milena, Paul Argho Aninda, Panek Kinga, Czechowska Ewa, Rychłowski Michał, Królicka Aleksandra, Zieliński Jacek, Gabrielsson Susanne, Pikuła Michał, Trzeciak Magdalena, Ogg Graham S., Gutowska-Owsiak Danuta. Excess filaggrin in keratinocytes is removed by extracellular vesicles to prevent premature death and this mechanism can be hijacked by Staphylococcus aureus in a TLR2-dependent fashion. Journal of Extracellular Vesicles 2023, 12: 12335 (doi: 10.1002/jev2.12335)

2. Urbiola Salvador Victor, Jabłońska Agnieszka, Miroszewska Dominika, Huang Qianru , Duzowska Katarzyna, Drężek-Chyła Kinga, Zdrenka Marek, Śrutek Ewa, Szylberg Łukasz, Jankowski Michał, Bała Dariusz, Zegarski Wojciech, Nowikiewicz Tomasz, Makarewicz Wojciech, Adamczyk Agnieszka, Ambicka Aleksandra, Przewoźnik Marcin, Harazin-Lechowicz Agnieszka, Ryś Janusz, Filipowicz Natalia, Piotrowski Arkadiusz, Dumanski Jan P. , Li Bin, Chen Zhi Jane. Plasma protein changes reflect colorectal cancer development and associated inflammation. Frontiers in Oncology 2023, 13: 1158261 (doi: 10.3389/fonc.2023.1158261)

3. Paul Argho Aninda, Szulc Natalia, Kobiela Adrian, Brown Sara J., Pokrzywa Wojciech, Gutowska-Owsiak Danuta. In silico analysis of the profilaggrin sequence indicates alterations in the stability, degradation route, and intracellular protein fate in filaggrin null mutation carriers. Frontiers in Molecular Biosciences 2023, 10:  (doi: 10.3389/fmolb.2023.1105678)

4. Cázares-Olivera Mariana, Miroszewska Dominika, Hu Lili, Kowalski Jacek, Jaakkola Ulla-Marjut, Salminen Seppo, Li Bin, Yatkin Emrah, Chen Zhi Jane. Animal unit hygienic conditions influence mouse intestinal microbiota and contribute to T-cell-mediated colitis. Experimental Biology and Medicine 2022, 247(19): 1752-1763 (doi: 10.1177/15353702221113826)

5. Kobiela Adrian, Frąckowiak Joanna E., Biernacka Anna, Hovhannisyan Lilit, Bogucka Aleksandra E., Panek Kinga, Paul Argho Aninda, Łukomska Joanna, Wang Xinwen, Giannoulatou Eleni, Królicka Aleksandra, Zielinski Jacek, Deptula Milena, Pikula Michal, Gabrielsson Susanne, Ogg Graham S., Gutowska-Owsiak Danuta. Exposure of keratinocytes to Candida albicans in the context of atopic milieu induces changes in the surface glycosylation pattern of small extracellular vesicles to enhance their propensity to interact with inhibitory Siglec receptors. Frontiers in Immunology 2022, 13: 884530 (doi: 10.3389/fimmu.2022.884530)

6. Urbiola Salvador Victor, Miroszewska Dominika, Jabłońska Agnieszka, Qureshi Talha, Chen Zhi Jane. Proteomics approaches to characterize the immune responses in cancer. Biochimica et Biophysica Acta-Molecular Cell Research 2022, 1869(8): 119266 (doi: 10.1016/j.bbamcr.2022.119266)

7. Le Menn Gwenaëlle, Jabłońska Agnieszka, Chen Zhi Jane. The effects of post-translational modifications on Th17/Treg cell differentiation. Biochimica et Biophysica Acta-Molecular Cell Research 2022, 1869: 119223 (doi: 10.1016/j.bbamcr.2022.119223)

8. Podobas Ewa Izabela, Gutowska-Owsiak Danuta, Moretti Sébastien, Poznański Jarosław, Kulińczak Mariusz, Grynberg Marcin, Gruca Aleksandra, Bonna Arkadiusz, Płonka Dawid, Ogg Graham, Bal Wojciech. Ni2+-Assisted Hydrolysis May Affect the Human Proteome; Filaggrin Degradation Ex Vivo as an Example of Possible Consequences. Frontiers in Molecular Biosciences 2022, 9: 828674 (doi: 10.3389/fmolb.2022.828674)

9. Rucinski Antoni, Biernacka Anna, Schulte Reinhard W. Applications of nanodosimetry in particle therapy planning and beyond. Physics in Medicine and Biology 2021, 66(24) (doi: 10.1088/1361-6560/ac35f1)

10. Hovhannisyan Lilit, Czechowska Ewa, Gutowska-Owsiak Danuta. The Role of Non-Immune Cell-Derived Extracellular Vesicles in Allergy. Frontiers in Immunology 2021, 12: 702381 (doi: 10.3389/fimmu.2021.702381)

11. Gutowska-Owsiak Danuta, Podobas Ewa I., Eggeling Christian, Ogg Graham S., de la Serna Jorge Bernardino. Addressing differentiation in live human keratinocytes by assessment of membrane packing order. Frontiers in Cell and Developmental Biology 2020, 8: 573230 (doi: 10.3389/fcell.2020.573230)
     
12. Nedoszytko Bogusław, Reszka Edyta, Gutowska-Owsiak Danuta, Trzeciak Magdalena, Lange Magdalena, Jarczak Justyna, Niedoszytko Marek, Jablonska Ewa, Romantowski Jan, Strapagiel Dominik, Skokowski Jarosław, Siekierzycka Anna, Nowicki Roman J, Dobrucki Iwona T., Zaryczańska Anna, Kalinowski Leszek. Genetic and epigenetic aspects of atopic dermatitis. International Journal of Molecular Sciences 2020, 21:6484 (doi:10.3390/ijms21186484)
     
13. Chen Yi-Ling, Gomes Tomas, Hardman Clare S., Vieira Braga Felipe A., Gutowska-Owsiak Danuta, Salimi Maryam, Gray Nicki, Duncan David A., Reynolds Gary, Johnson David, Salio Mariolina, Cerundolo Vincezo, L. Barlow Jilian L., McKenzie Andrew N.J., Teichmann Sarah A., Haniffa Muzlifah, Ogg Graham.  DC during acute sterile skin inflammation. Journal of Experimental Medicine 2020, 217 (3): e20190811 (doi: 10.1084/jem.20190811)
     
14. Chen Yi-Ling, Gutowska-Owsiak Danuta, Hardman Clare S., Westmoreland Melanie, MacKenzie Teena, Cifuentes Liliana, Waithe Dominic, Lloyd-Lavery Antonia, Marquette Allison, Londei Marco, Ogg Graham. Proof-of-concept clinical trial of etokimab shows a key role for IL-33 in atopic dermatitis pathogenesis. Science Translational Medicine 2019, 11 (515): pii: eaax2945 (doi: 10.1126/scitranslmed.aax2945)
     
15. Gutowska-Owsiak Danuta, Bernardino de La Serna Jorge, Fritzsche Marco, Naeem Aishath, Podobas Ewa I., Leeming Michael, Colin-York Huw, O’Shaughnessy Ryan, Eggeling Christian, Ogg Graham S. Orchestrated control of filaggrin–actin scaffolds underpins cornification. Cell Death & Disease 2018, 9: 412 (doi: 10.1038/s41419-018-0407-2)