Laboratory of Evolutionary Biochemistry

Research areas:

• Molecular mechanisms of iron-sulfur cluster biogenesis
• The role of the Hsp70 machinery in mitochondrial iron-sulfur cluster biogenesis and maintenance and propagation of mitochondrial genome
• Molecular evolution of the Hsp70 systems
• Molecular and biochemical evolution of proteins

Laboratory of Evolutionary Biochemistry

Department of Molecular and Cellular Biology
Intercollegiate Faculty of Biotechnology UG&MUG
Abrahama 58 Street, 80-307 Gdańsk, Poland

Office: Ewa Kogut, Stanisława Urbańska,
Tel.:+ 48 58 523 6427, +48 58 523 6421

STAFF LIST

Head: Prof. Jarosław Marszałek [link]
tel.: +48 58 523 6313 [e-mail]

List of Employees and PhD students:

Dsc. Rafał Dutkiewicz [link]
tel.: +48 58 523 6351 [e-mail]

MSc Eng. Bartłomiej Tomiczak [link]
tel.: +48 58 523 6326 [e-mail]

MSc Małgorzata Kleczewska
tel.: +48 58 523 6326 [email], 
MSc Marcin Jeleń
tel.: +48 58 523 6326 [email]
MSc Mielna Stolarska
tel.: +48 58 523 6326 [email]
MSc Aneta Grabińska-Rogala
tel.: +48 58 523 6326 [email]
MSc Igor Grochowina
tel.: +48 58 523 6326 [email]
MSc Marcin Pitek
tel.: +48 58 523 6326 [email]
MSc Katarzyna Kalinowska
tel.: +48 58 523 6326 [email]

 

1. Marszałek Jarosław, Craig Elizabeth A.. Interaction of client—the scaffold on which FeS clusters are build—with J-domain protein Hsc20 and its evolving Hsp70 partners. Frontiers in Molecular Biosciences 2022, 9: 1034453 (doi: 10.3389/fmolb.2022.1034453)

2. Srour Batoul, Gervason Sylvain, Hoock Maren Hellen, Monfort Beata, Want Kristian, Larkem Djabir, Trabelsi Nadine, Landrot Gautier, Zitolo Andrea, Fonda Emiliano, Etienne Emilien, Gerbaud Guillaume, Müller Christina Sophia, Oltmanns Jonathan, Gordon Jesse B., Yadav Vishal, Kleczewska Małgorzata, Jeleń Marcin, Toledano Michel B., Dutkiewicz Rafał, Goldberg David P., Schünemann Volker, Guigliarelli Bruno, Burlat Bénédicte, Sizun Christina, D’Autréaux Benoit. Iron Insertion at the Assembly Site of the ISCU Scaffold Protein Is a Conserved Process Initiating Fe−S Cluster Biosynthesis. Journal of the American Chemical Society 2022, 144(38): 17496–17515 (doi: 10.1021/jacs.2c06338)

3. Uzarska Marta A ., Grochowina Igor, Sołdek Joanna, Jeleń Marcin, Schilke Brenda, Marszałek Jarosław, Craig Elizabeth A., Dutkiewicz Rafał. During FeS-cluster biogenesis ferredoxin and frataxin use overlapping bindings sites on yeast cysteine desulfurase Nfs1. Journal of Biological Chemistry 2022, 298(2): 101570 (doi: 10.1016/j.jbc.2022.101570)

4. Piróg Artur, Cantini Francesca, Nierzwicki Łukasz, Obuchowski Igor, Tomiczek Bartłomiej, Czub Jacek, Liberek Krzysztof. Two Bacterial Small Heat Shock Proteins, IbpA and IbpB, Form a Functional Heterodimer. Journal of Molecular Biology 2021, 433: 167054 (doi: 10.1016/j.jmb.2021.167054)

5. Węgrzyn Katarzyna, Zabrocka Elżbieta, Bury Katarzyna, Tomiczek Bartłomiej, Wieczór Miłosz, Czub Jacek, Uciechowska-Kaczmarzyk Urszula, Moreno-del Alamo Maria, Walkow Urszula, Grochowina Igor, Dutkiewicz Rafał, Bujnicki Janusz M., Giraldo Rafael, Konieczny Igor. Defining a novel domain that provides an essential contribution to site-specific interaction of Rep protein with DNA. Nucleic Acids Research 2021, 49(6): 3394-3408 (doi: 10.1093/nar/gkab113)

6. Muehlenhoff Ulrich, Braymer Joseph J., Christ, Stefan, Rietzschel Nicole, Uzarska Marta A., Weiler Benjamin D., Lill Roland. Glutaredoxins and iron-sulfur protein biogenesis at the interface of redox biology and iron metabolism. Biological Chemistry 2020, 401(12): 1407-1428 (doi: 10.1515/hsz-2020-0237)

7. Tomiczek Bartłomiej, Delewski Wojciech, Nierzwicki Łukasz, Stolarska Milena, Grochowina Igor, Schilke Brenda, Dutkiewicz Rafał, Uzarska Marta A., Ciesielski Szymon J., Czub Jacek, Craig Elizabeth A., Marszałek Jarosław. Two-step mechanism of J-domain action in driving Hsp70 function. PLOS Computational Biology 2020, 16(6): e1007913 (doi: 10.1371/journal.pcbi.1007913)

8. Kleczewska Małgorzata, Grabińska Aneta, Jeleń Marcin, Stolarska Milena, Schilke Brenda, Marszałek Jarosław, Craig Elizabeth A., Dutkiewicz Rafał. Biochemical Convergence of Mitochondrial Hsp70 System Specialized in Iron–Sulfur Cluster Biogenesis. International Journal of Molecular Sciences 2020, 21(9): 3326 (doi: 10.3390/ijms21093326)

9. Mróz Dagmara, Wyszkowski Hubert, Szablewski Tomasz, Zawieracz Katarzyna, Dutkiewicz Rafał, Bury Katarzyna, B., Wortmann Saskia, Wevers Ron A., Ziętkiewicz Szymon. CLPB (caseinolytic peptidase B homolog), the first mitochondrial protein refoldase associated with human disease. Biochimica et Biophysica Acta (BBA) - General Subjects 2020, 1864 (4): 129512 (doi: 10.1016/j.bbagen.2020.129512)

10. Obuchowski Igor, Piróg Artur, Stolarska Milena, Tomiczek Bartłomiej, Liberek Krzysztof. Duplicate divergence of two bacterial small heat shock proteins reduces the demand for Hsp70 in refolding of substrates. PLoS Genet  2019, 15(10): e1008479 (doi: 10.1371/journal.pgen.1008479)

11. Shrestha Om Kumar, Sharma Ruchika, Tomiczek Bartłomiej, Lee Woonghee, Tonelli Marco,Cornilescu Gabriel, Stolarska Milena, Nierzwicki Lukasz, Czub Jacek, Markley John L., Marszalek Jaroslaw , b Szymon J. Ciesielski , Elizabeth A. Craig . Structure and evolution of the 4-helix bundle domain of Zuotin, a J-domain protein co-chaperone of Hsp70. PLoS One 2019, 14(5): e0217098 (doi: 10.1371/journal.pone.0217098)

12. Philippe Herve, Poustka Albert J., Chiodin Marta, Hoff Katharina J., Dessimoz Christophe, Tomiczek Bartłomiej, Schiffer Philipp H., Mueller Steven, Domman Daryl, Horn Matthias, Kuhl Heiner, Timmermann Bernd, Satoh Noriyuki, Hikosaka-Katayama Tomoe, Nakano Hiroaki, Rowe Matthew L., Elphick Maurice R., Thomas-Chollier Morgane, Hankeln Thomas, Mertes Florian, Wallberg Andreas, Rast Jonathan P., Copley Richard R., Martinez Pedro, Telford Maximilian J. Mitigating Anticipated Effects of Systematic Errors Supports Sister-Group Relationship between Xenacoelomorpha and Ambulacraria. Current Biology 2019, 29(11): 1818-1826 (doi: 10.1016/j.cub.2019.04.009)

13. Altenhoff Adrian M., Levy Jeremy, Zarowiecki Magdalena, Tomiczek Bartłomiej, Vesztrocy Alex Warwick, Dalquen Daniel A., Mueller Steven, Telford Maximilian J., Glover Natasha M., Dylus David, Dessimoz Christophe. OMA standalone: orthology inference among public and custom genomes and transcriptomes. Genome Research 2019, 29(7): 1152-1163  (doi: 10.1101/gr.243212.118)

14. Kampinga Harm H. Andreasson Claes, Barducci Alessandro, Cheetham Michael E., Cyr Douglas, Emanuelsson Cecilia, Genevaux Pierre, Gestwicki Jason E., Goloubinoff Pierre, Huerta-Cepas Jaime, Kirstein Janine, Liberek Krzysztof, Mayer Matthias P., Nagata Kazuhiro, Nillegoda Nadinath B.,Pulido Pablo; Ramos Carlos; De los Rios Paolo; Rospert Sabine, Rosenzweig Rina, Sahi Chandan, Taipale Mikko, Tomiczek Bratłomiej, Ushioda Ryo, Young Jason C., Zimmermann Richard, Zylicz Alicja, Zylicz Maciej, Craig Elizabeth A., Marszałek Jarosław. Function, evolution, and structure of J-domain proteins. Cell Stress & Chaperones 2019, 24(1): 7-15 (doi: 10.1007/s12192-018-0948-4)

15. Dutkiewicz Rafał, Nowak Małgorzata. Molecular chaperones involved in mitochondrial iron–sulfur protein biogenesis. JBIC Journal of Biological Inorganic Chemistry 2018, 23(4): 569-579 (doi: 10.1007/s00775-017-1504-x)

16. Dutkiewicz Rafał, Nowak Malgorzata, Craig Elizabeth A, Marszałek Jaroslaw. Chapter Six - Fe–S Cluster Hsp70 Chaperones: The ATPase Cycle and Protein Interactions. Methods in Enzymology 2017, 595: 161-184 (doi: 10.1016/bs.mie.2017.07.004)

17. Craig Elizabeth A., Marszałek Jarosław. How do J-Proteins get Hsp70 to do So many different things? Trends in Biochemical Sciences 2017, 42(5): 355-368 (doi:  10.1016/j.tibs.2017.02.007 )

18. Delewski Wojciech, Paterkiewicz Bogumiła, Manicki Mateusz, Schilke Brenda, Tomiczek Bartosz, Ciesielski Szymon J. , Nierzwicki Łukasz, Czub Jacek, Dutkiewicz Rafał, Craig Elizabeth A., Marszałek Jarosław. Iron-Sulfur cluster biogenesis chaperones: evidence for emergence of mutational robustness of a highly specific protein-protein interaction. Molecular Biology and Evolution 2016, 33(3): 643-656 (doi: 10.1093/molbev/msv254).

19. Lilla Roland, Dutkiewicza Rafał, Freiberta Sven A., Heidenreicha Torsten, Mascarenhasa Judita, Netza Daili J., Paula Viktoria D., Pierika Antonio J., Richtera Nadine, Stümpfiga Martin, Srinivasana Vasundara, Stehlinga Oliver, Mühlenhoff Ulrich. The role of mitochondria and the CIA machinery in the maturation of cytosolic and nuclear iron-sulfur proteins. European Journal of Cell Biology 2015, 94(7-9): 280-291 (doi: 10.1016/j.ejcb.2015.05.002).

20. Manicki Mateusz, Majewska Julia, Ciesielski Szymon, Schilke Brenda, Błenska Anna, Kominek Jacek, Marszałek Jarosław, Craig Elizabeth A., Dutkiewicz Rafał. Overlapping binding sites of the frataxin homologue assembly factor and the heat shock protein 70 transfer factor on the Isu iron-sulfur cluster scaffold protein. Journal of Biological Chemistry 2014, 289(44): 30268-30278 (doi: 10.1074/jbc.M114.596726).

21. Ciesielski Grzegorz L., Płotka Magdalena, Manicki Mateusz, Schilke Brenda A., Dutkiewicz Rafał, Sahi Chandan, Marszałek Jarosław, Craig Elizabeth A. Nucleoid localization of Hsp40 Mdj1 is important for its function in maintenance of mitochondrial DNA. Biochimica et Biophysica Acta-Molecular Cell Research 2013, 1833(10): 2233-2243 (doi: 10.1016/j.bbamcr.2013.05.012).

22. Kominek Jacek, Marszałek Jarosław, Neuvéglise Cecile, Craig Elizabeth A., Williams Barry L. The Complex Evolutionary Dynamics of Hsp70s: A Genomic and Functional Perspective. Genome Biology and Evolution 2013, 5(12): 2460-2477 (doi: 10.1093/gbe/evt192).

23. Majewska Julia, Ciesielski Szymon J., Schilke Brenda, Kominek Jacek, Błeńska Anna, Delewski Wojciech, Song Ji-Yoon, Marszałek Jarosław, Craig Elizabeth A., Dutkiewicz Rafał. Binding of the chaperone jac1 protein and cysteine desulfurase nfs1 to the iron-sulfur cluster scaffold isu protein is mutually exclusive. Journal of Biological Chemistry 2013, 288(40): 29134-29142 (doi: 10.1074/jbc.M113.503524).

24. Sahi Chandan, Kominek Jacek, Ziegelhoffer Thomas, Yu Hyun-Young, Baranowski Maciej, Marszałek Jarosław, Craig Elizabeth A. Sequential Duplications of an Ancient Member of the DnaJ-Family Expanded the Functional Chaperone Network in the Eukaryotic Cytosol. Molecular Biology and Evolution 2013, 30(5): 985-998 (doi: 10.1093/molbev/mst008).

25. Uzarska Marta A., Dutkiewicz Rafał, Freibert Sven-Andreas, Lill Roland, Muehlenhoff Ulrich. The mitochondrial Hsp70 chaperone Ssq1 facilitates Fe/S cluster transfer from Isu1 to Grx5 by complex formation. Molecular Biology of the Cell 2013, 24(12): 1830-1841 (doi: 10.1091/mbc.E12-09-0644).

26. Ciesielski Szymon J., Schilke Brenda A., Osipiuk Jerzy, Bigelow Lance, Mulligan Rory, Majewska Julia, Joachimiak Andrzej, Marszałek Jarosław, Craig Elizabeth A., Dutkiewicz Rafał. Interaction of J-Protein Co-Chaperone Jac1 with Fe-S Scaffold Isu Is Indispensable In Vivo and Conserved in Evolution. Journal of Molecular Biology 2012, 417(1-2): 1-12 (doi: 10.1016/j.jmb.2012.01.022).

27. Song J.-Y., Marszałek J., Craig E. Cysteine desulfurase Nfs1 and Pim1 protease control levels of Isu, the Fe-S cluster biogenesis scaffold. Proceedings of the National Academy of Sciences of the United States of America 2012, 109(26): 10370-10375 (doi: 10.1073/pnas.1206945109).

28. Craig E.A., Marszałek J. Hsp70 Chaperones. W: Encyclopedia of Life Sciences (ELS). John Wiley & Sons, Ltd: Chichester 2011, publication online 15.03.2011: 1-8.

29. Hayashi M., Schilke B., Marszałek J., Williams B., Craig E. A. Ancient gene duplication provided a key molecular step for anaerobic growth of baker's yeast. Molecular Biology and Evolution 2011, 28(7): 2005-2017 (doi: 10.1093/molbev/msr019).

30. Nidzworski Dawid, Kuźbicki Marek, Kowalczyk Izabela, Matusiak Krzysztof, Przybyłowski Michał, Kipiel Kamil, Muzalewska Magdalena, Ciesielski Szymon. Bio Bussines School. Przewodnik po komercjalizacji wyników w branży Life Science. Żary 2010 (ISBN 978-83-61315-05-6) 1-54.

31. Pukszta Sebastian, Schilke Brenda, Dutkiewicz Rafał, Kominek Jacek, Moczulska Kaja, Stępień Barbara, Reitenga Krista G., Bujnicki Janusz M., Williams Barry, Craig Elizabeth A., Marszałek Jarosław. Co-evolution-driven switch of J-protein specificity towards an Hsp70 partner. EMBO Reports 2010, 11(5): 360-365 (doi: 10.1038/embor.2010.29).