Laboratory of Virus Molecular Biology | Intercollegiate Faculty of Biotechnology UG&MUG

Laboratory of Virus Molecular Biology

Last modified: 
2019, May 20 - 9:40am

Zakład Biologii Molekularnej Wirusów

PROFILE

Research areas:

  • Structure and functions of herpesvirus proteins
  • Mechanisms of viral cell-to-cell spread
  • Viral immune evasion strategies
  • Structure and functions of Hepatitis C virus (HCV) envelope glycoproteins
  • Construction of viral vectors for research and vaccine applications
  • Production of recombinant viral protein

About NCN project "How viruses fight for survival" prof. Krystyna Bieńkowska-Szewczyk

RESEARCH TEAM

Laboratory of Virus Molecular Biology

Intercollegiate Faculty of Biotechnology UG&MUG
Abrahama 58 Street, 80-307 Gdańsk, Poland

Office:
Elżbieta Brycka [e-mail]
Aleksandra Krejmer [e-mail]

Ph.: +48 58 523 6335

Website: wirusologia-mwb.cba.pl

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STAFF LIST

Head: Prof. Krystyna Bieńkowska-Szewczyk 
tel.: +48 58 523 6381 [e-mail]

List of Employees and PhD students:

dr Alicja Chmielewska 
tel.: +48 58 523 6385 [e-mail]
dr Andrea Lipińska 
tel.: +48 58 523 6383 [e-mail]
dr Małgorzata Rychłowska 
tel.: +48 58 523 6383 [e-mail]
dr Michał Rychłowski 
[e-mail]
dr Katarzyna Grzyb
tel.+48 58 523 6338 [e-mail]

mgr Malgorzata Graul
tel.+48 58 523 6387 [e-mail]
mgr Weronika Hoffmann
tel.: +48 58 523 6336 [e-mail]

Małgorzata Piotrowska 
tel.: +48 58 523 6336 [e-mail]

mgr Natalia Derewońko
tel.: +48 58 523 6382 [e-mail]
mgr Kinga Grabowska
tel.: +48 58 523 6386 [e-mail]
mgr Magdalena Wąchalska
tel.: +48 58 523 6386
mgr Paulina Gach
tel.: +48 58 523 6386
mgr Anna Czarnota
tel.: +48 58 523 6386

PUBLICATION

  1. Borowik Agnieszka, Banasiuk Rafał, Derewonko NataliaRychłowski Michał, Krychowiak-Maśnicka Marta, Wyrzykowski Dariusz, Ziąbka Magdalena, Woziwodzka Anna, Królicka Aleksandra, Piosik Jacek. Interactions of newly synthesized platinum nanoparticles with ICR-191 and their potential application. Scientific Reports 2019, 9: 4987 (doi: 10.1038/s41598-019-41092-6)
  2. Kowalski Szymon, Wyrzykowski Dariusz, Hac Stanislaw, Rychlowski Michal, Radomski Marek W, Inkielewicz-Stepniak Iwona. New oxidovanadium(IV) coordination complex containing 2-methylnitrilotriacetate ligands induces cell cycle arrest and autophagy in human pancreatic ductal adenocarcinoma cell lines. International Journal of Molecular Sciences 2019, 20(2); 261 (doi: 10.3390/ijms20020261)

  3. Graul Malgorzata, Kisielnicka Edyta, Rychłowski Michał, Verweij Marieke C., Tobler Kurt), Ackermann Mathias, Wiertz Emmanuel J. H. J., Bieńkowska-Szewczyk Krystyna, Lipińska Andrea D. Transmembrane regions of bovine herpesvirus 1-encoded UL49.5 and glycoprotein M regulate complex maturation and ER-Golgi trafficking. Journal of General Virology 2019, 100 (3): 497-510 (doi: 10.1099/jgv.0.001224)

  4. Karska Natalia, Graul Małgorzata, Sikorska Emilia, Zhukov Igor, Ślusarz Magdalena J, Kasprzykowski Franciszek, Lipińska Andrea D, Rodziewicz-Motowidlo Sylwia. Structure determination of UL49.5 transmembrane protein from bovine herpesvirus 1 by NMR spectroscopy and molecular dynamics. Biochimica et Biophysica Acta-Biomembranes 2019, 1861 (5): 926-938 (doi: 10.1016/j.bbamem.2019.02.005)

  5. Mieszkowska Magdalena, Piasecka Dominika, Potemski Piotr, Debska-Szmich Sylwia, Rychłowski Michał, Kordek Radzisław, Sądej Rafał, Romanska Hanna M.. Tetraspanin CD151 impairs heterodimerization of ErbB2/ErbB3 in breast cancer cells. Translational Research 2018, 207: 44-55 (doi: 10.1016/j.trsl.2018.12.007)

  6. Panasiuk Mirosława, Rychłowski MichałDerewonko Natalia, Bieńkowska-Szewczyk Krystyna. Tunneling Nanotubes as a Novel Route of Cell-to-Cell Spread of Herpesviruses. Journal of Virology 2018; 9(10): e00090-18 (doi: 10.1128/JVI.00090-18)

  7. Fila Grzegorz, Krychowiak Marta, Rychlowski Michał, Bielawski Krzysztof, Grinholc Mariusz. Antimicrobial blue light photoinactivation of Pseudomonas aeruginosa: Quorum sensing signaling molecules, biofilm formation and pathogenicity. Journal of Biophotonics 2018, e201800079 (doi: 10.1002/jbio.201800079)

  8. Krapchev Vasil N., Rychłowska Małgorzata, Chmielewska Alicja, Zimmer KarolinaPatel Arvind, Bienkowska-Szewczyk KrystynaRecombinant Flag-tagged E1E2 glycoproteins from three hepatitis C virus genotypes are biologically functional and elicit cross-reactive neutralizing antibodies in mice. Virology 2018, 519: 33-41 (doi: 10.1016/j.virol.2018.03.026).

  9. Wenta Tomasz, Zurawa-Janicka Dorota, Rychłowski Michał, Jarzab Mirosław, Glaza Przemysław, Lipińska AndreaBieńkowska-Szewczyk Krystyna, Herman-Antosiewicz Anna, Skorko-Gloneka Joanna, Lipińska Barbara. HtrA3 is a cellular partner of cytoskeleton proteins and TCP1α chaperonin. Journal of Proteomics 2018, 177: 88-111 (doi: 10.1016/j.jprot.2018.02.022)

  10. Brzozowska Agnieszka, Lipińska Andrea D., Derewońko Natalia, Lesiak Dorota, Rychłowski Michał, Rąbalski Łukasz, Bieńkowska-Szewczyk Krystyna. Inhibition of apoptosis in BHV-1-infected cells depends on Us3 serine/threonine kinase and its enzymatic activity. Virology 2018, 513: 136-145 (doi: 10.1016/j.virol.2017.09.029)

  11. Rogacka Dorota, Audzeyenka Irena, Rychłowski Michał, Rachubik Patrycja, Szrejder Maria, Angielski Stefan, Piwkowska Agnieszka. Metformin overcomes high glucose-induced insulin resistance of podocytes by pleiotropic effects on SIRT1 and AMPK. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 2018, 1864: 115-125 (doi: 10.1016/j.bbadis.2017.10.014)

  12. Gabriel Iwona, Rychlowski Michał. Consequences of lysine auxotrophy for Candida albicans adherence and biofilm formation. Acta Biochimica Polonica 2017, 64(2): 323-329 (doi: 10.18388/abp.2016_1427)

  13. Rogacka Dorota, Audzeyenka Irena, Rachubik Patrycja, Rychlowski Michal, Kasztan Malgorzata, Jankowski Maciej, Angielski Stefan, Piwkowska Agnieszka. Insulin increases filtration barrier permeability via TRPC6-dependent activation of PKGI alpha signaling pathways. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 2017, 1863(6):1312-1325 (doi: 10.1016/j.bbadis.2017.03.002)

  14. Audzeyenka IrenaRogacka Dorota, Piwkowska Agnieszka, Rychlowski Michal, Bierla, Joanna B, Czarnowska Elzbieta, Angielski Stefan, Jankowski Maciej. Reactive oxygen species are involved in insulin-dependent regulation of autophagy in primary rat podocytes. International Journal of Biochemistry and Cell Biology 2016, 75: 23-33 (doi: 10.1016/j.biocel.2016.03.015).

  15. Grzyb Katarzyna, Czarnota Anna, Brzozowska Agnieszka, Cieślik Anna, Rąbalski Łukasz, Tyborowska Jolanta, Bieńkowska-Szewczyk Krystyna. Immunogenicity and functional characterization of Leishmania-derived hepatitis C virus envelope glycoprotein complex. Scientific Reports 2016, 6: 30627 (doi: 10.1038/srep30627)

  16. Wachlewska Magda, Danijela Koppers-Lalic, Monique van Eijndhoven , Michiel Pegtel , Albert A. Geldof , Lipinska Andrea D., R. Jeroen van Moorselaar, Irene V. Bijnsdorp. Protein Complexes in Urine Interfere with Extracellular Vesicle Biomarker Studies. Journal of Circulating Biomarkers 2016, 5:4 (doi: 10.5772/62579)

  17. Koralewska Natalia, Hoffmann Weronika, Pokornowska Maria, Milewski Marek, Lipinska Andrea, Bienkowska-Szewczyk Krystyna, Figlerowicz Marek, Kurzynska-Kokorniak Anna. How short RNAs impact the human ribonuclease Dicer activity: putative regulatory feedback-loops and other RNA-mediated mechanisms controlling microRNA processing. Acta Biochimica Polonica 2016, 63(4): 773-783 (doi: 10.18388/abp.2016_1339)

  18. Kurzyńska-Kokorniak  Anna, Pokornowska  Maria, Koralewska  Natalia, Hoffmann Weronika, Bieńkowska-Szewczyk Krystyna, Figlerowicz, Marek. Revealing a new activity of the human Dicer DUF283 domain in vitro. Scientific Reports 2016,  6: 23989-23989 (doi: 10.1038/srep23989)

  19. Ogryzek Małgorzata, Chylewska Agnieszka, Królicka Aleksandra, Banasiuk Rafał, Turecka Katarzyna, Lesiak Dorota, Nidzworski Dawid, Makowski Mariusz. Coordination chemistry of pyrazine derivatives analogues of PZA: design, synthesis,characterization and biological activity. RSC Advances 2016, 6: 52009-52025 (doi: 10.1039/C6RA03068H).

  20. Czarnota AnnaTyborowska Jolanta, Peszyńska-Sularz Grażyna, Gromadzka BeataBieńkowska-Szewczyk KrystynaGrzyb Katarzyna. Immunogenicity of Leishmania-derived hepatitis B small surface antigen particles exposing highly conserved E2 epitope of hepatitis C virus. Microbial Cell Factories 201615: 62  (doi: 10.1186/s12934-016-0460-4)

  21. Gołuński Grzegorz, Borowik Agnieszka, Derewońko Natalia, Kawiak Anna, Rychłowski Michał, Woziwodzka Anna, Piosik Jacek. Pentoxifylline as a modulator of anticancer drug doxorubicin. Part II: Reduction of doxorubicin DNA binding and alleviation of its biological effects. Biochimie 2016, 123: 95-102 (doi: 10.1016/j.biochi.2016.02.003)

  22. Gołuński Grzegorz, Borowik Agnieszka, Lipińska Andrea, Romanik Monika, Derewońko Natalia, Woziwodzka Anna, Piosik Jacek. Pentoxifylline affects idarubicin binding to DNA. Bioorganic Chemistry 2016, 65: 118-125 (doi: 10.1016/j.bioorg.2016.02.005)

  23. Chmielewska Alicja M.Rychłowska Małgorzata, Król Ewelina, Solarz KarolinaBieńkowska-Szewczyk Krystyna. Wirusowe zapalenie wątroby typu C - nowe metody leczenia i zapobiegania [Novel methods of hepatitis C treatment and prevention]. Postępy Higieny i Medycyny Doświadczalnej 2015, 69: 946-963 (doi: 10.5604/17322693.1165197)

  24. Niedźwiecka Natalia, Grzyb Katarzyna, Nona-Mołdawa Agnieszka, Gronczewska Jadwiga, Skorkowski Edward F. Purification and stability of octameric mitochondrial creatine kinase isoform from herring (Clupea harengus) organ of vision. Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology 2015, 185: 16-23 (doi: 10.1016/j.cbpb.2015.03.002).

  25. Urbanowicz Richard A., Lacek Krzysztof, Lahm Armin, Bieńkowska-Szewczyk Krystyna, Ball Jonathan K., Nicosia Alfredo, Cortese Riccardo, Pessi Antonello. Cholesterol conjugation potentiates the antiviral activity of an HIV immunoadhesin. Journal of Peptide Science 2015, 21(9): 743-749 (doi: 10.1002/psc.2802).

  26. van den Wollenberg D. J. M. , Dautzenberg I. J. C., Ros W., Lipińska A. D., van den Hengel S. K., Hoeben R. C. Replicating reoviruses with a transgene replacing the codons for the head domain of the viral spike. Gene Therapy 2015, 22(3): 267-279 (doi: 10.1038/gt.2014.126).

  27. Moskot Marta, Jakóbkiewicz-Banecka Joanna, Kloska Anna, Smolińska Elwira, Mozolewski Paweł, Malinowska Marcelina, Rychłowski Michał, Banecki Bogdan, Węgrzyn Grzegorz, Gabig-Cimińska Magdalena. Modulation of expression of genes involved in glycosaminoglycan metabolism and lysosome biogenesis by flavonoids. Scientific Reports 2015, 5: art. no 9378 (1-13) (doi: 10.1038/srep09378).

  28. Chmielewska Alicja M., Naddeo Mariarosaria, Capone Stefania, Ammendola Virginia, Hu Ke, Meredith Luke, Verhoye Lieven, Rychłowska Małgorzata, Rappuoli Rino, Ulmer Jeffrey B., Colloca Stefano, Nicosia Alfredo, Cortese Riccardo, Leroux-Roels Geert, Balfe Peter, Bieńkowska-Szewczyk Krystyna, Meuleman Philip, McKeating Jane A., Folgori Antonella. Combined adenovirus vector and hepatitis C virus envelope protein prime-boost regime elicits T cell and neutralizing antibody immune responses. Journal of Virology 2014, 88(10): 5502-5510 (doi: 10.1128/JVI.03574-13).

  29. Czaplińska Dominika, Turczyk Łukasz, Grudowska Alicja, Mieszkowska Magdalena, Lipińska Andrea D., Składanowski Andrzej C., Żaczek Anna J., Romańska Hanna M., Sądej Rafał. Phosphorylation of RSK2 at Tyr529 by FGFR2-p38 enhances human mammary epithelial cells migration. Biochimica et Biophysica Acta-Molecular Cell Research 2014, 1843(11): 2461-2470 (doi: 10.1016/j.bbamcr.2014.06.022).

  30. Król Ewelina, Pastuch-Gawolek Gabriela, Nidzworski Dawid, Rychłowski Michał, Szeja Wiesław, Grynkiewicz Grzegorz, Szewczyk Bogusław. Synthesis and antiviral activity of a novel glycosyl sulfoxide against classical swine fever virus. Bioorganic & Medicinal Chemistry 2014, 22(9): 2662-2670 (doi: 10.1016/j.bmc.2014.03.027).
  31. Król Ewelina, Rychłowska Małgorzata, Szewczyk Bogusław. Antivirals - current trends in fighting influenza. Acta Biochimica Polonica 2014, 61(3): 495-504 (pdf).

  32. Lacek Krzysztof, Bauer Berthilde, Bieńkowska-Szewczyk Krystyna, RzihaHanns-Joachim. Orf virus (ORFV) ANK-1 protein mitochondrial localization is mediated by ankyrin repeat motifs. Virus Genes 2014, 49(1): 68-79 (doi: 10.1007/s11262-014-1069-5).

  33. Lacek Krzysztof, Urbanowicz Richard A., Troise Fulvia, De Lorenzo Claudia, Severino Valeria, Di Maro Antimo, Tarr Alexander W., Ferrara Francesca, Ploss Alexander, Temperton Nigel, Ball Jonathan K., Nicosia Alfredo, Cortese Riccardo, Pessi Antonello. Dramatic potentiation of the antiviral activity of HIV antibodies by cholesterol conjugation. Journal of Biological Chemistry 2014, 2889(50): 35015-35028 (doi: 10.1074/jbc.M114.591826).

  34. Siedlecka-Kroplewska K., Szczerba A., Lipińska A., Slebioda T., Kmieć Z. 3-Fluoromethcathinone, a structural analog of mephedrone, inhibits growth and induces cell cycle arrest in HT22 mouse hippocampla cells. Journal of Physiology and Pharmacology 2014, 65(2): 241-246 (pdf).

  35. Szewczyk Bogusław, Bieńkowska-Szewczyk Krystyna, Król Ewelina. Introduction to molecular biology of influenza A viruses. Acta Biochimica Polonica 2014, 61(3): 397-401 (pdf).

  36. Kotlarz Agnieszka, Tukaj Stefan, Krzewski Konrad, Brycka Elżbieta, Lipińska Barbara. Human Hsp40 proteins, DNAJA1 and DNAJA2, as potential targets of the immune response triggered by bacterial DnaJ in rheumatoid arthritis. Cell stress & chaperones 2013, 18(5): 653-659.

  37. Król Ewelina, Wandzik Ilona, Gromadzka BeataNidzworski DawidRychłowska MałgorzataMatłacz MartaTyborowska JolantaSzewczyk Bogusław. Anti-influenza A virus activity of uridine derivatives of 2-deoxy sugars. Antiviral Research 2013, 100(1): 90-97.

  38. Lesiak DorotaBrzozowska AgnieszkaBieńkowska-Szewczyk KrystynaLipińska Andra D. US3 - wielofunkcyjna kinaza serynowo-treoninowa alfaherpeswirusów [US3-a multifunctional serine-threonine kinase of alphaherpesviruses]. Advances in Microbiology (Postępy Mikrobiologii) 2013, 52(4): 329-341 (pdf).
  39. Siedlecka-Kroplewska K., Jóżwik A., Bogusławski W., Woźniak M., Zauszkiewicz-Pawlak A., Spodnik J. H., Rychłowski M., Kmieć Z. Pterostilbene induces accumulation of autophagic vacuoles followed by cell death in HL60 human leukemia cells. Journal of Physiology and Pharmacology 2013, 64(5): 545-556.

  40. Krzyżanowska Dorota, Obuchowski Michał, Bikowski Mariusz, Rychłowski Michał, Jafra Sylwia. Colonization of potato rhizosphere by GFP-tagged Bacillus subtilis MB73/2, Pseudomonas sp. P482 and Ochrobactrum sp. A44 shown on large sections of root using enrichment sample preparation and confocal laser scanning microscopy. Sensors 2012, 12(12): 17608-17619.

  41. Lacek Krzysztof, Vercauteren Koen, Grzyb Katarzyna, Naddeo Mariarosaria, Verhoye Lieven, Słowikowski Marek Patryk, Fafi-Kremer Samira, Patel Arvind H., Baumert Thomas F., Folgori Antonella, Leroux-Roels Geert, Cortese Riccardo, Meuleman Philip, Nicosia Al. Novel human SR-BI antibodies prevent infection and dissemination of HCV in vitro and in humanized mice. Journal of Hepatology 2012, 57(1): 17-23.

  42. Rychłowska MałgorzataGromadzka BeataBieńkowska-Szewczyk KrystynaSzewczyk BogusławApplication of baculovirus-insect cell expression system for human therapy. Current Pharmaceutical Biotechnology 2011, 12(11): 1840-1849.

  43. Rychłowska Małgorzata, Owsianka Ania M., Foung Steven K. H., Dubuisson Jean, Bieńkowska-Szewczyk Krystyna, Patel Arvind H. Comprehensive linker-scanning mutagenesis of the hepatitis C virus E1 and E2 envelope glycoproteins reveals new structure-function relationships. Journal of General Virology 2011, 92(Pt 10): 2249-2261.

  44. Verweij M.C., Lipińska A. D., Koppers-Lalic D., van Leeuwen W.F., Cohen J.I., Kinchington P.R., Messaoudi I., Bieńkowska-Szewczyk K., Ressing M.E., Rijsewijk F.A.M., Wiertz E. J.H.J. The capacity of UL49.5 proteins to inhibit TAP is widely distributed among members of the genus Varicellovirus. Journal of Virology 2011, 85(5): 2351-2363.

  45. Verweij Marieke C., Lipińska Andrea D., Koppers-Lalic Danijela, Quinten Edwin, Funke Jessica, van Leeuwen Hans C., Bieńkowska-Szewczyk Krystyna, Koch Joachim, Ressing Maaike E., Wiertz Emmanuel J.H.J. Structural and functional analysis of the TAP-inhibiting UL49.5 proteins of varicelloviruses. Molecular Immunology2011, 48(15-16): 2038-2051.

  46. Adamiak Beata, Trybala Edward, Mardberg Kristina, Johansson Maria, Liljeqvist Jan-Ake, Olofsson Sigvard, Grabowska AgnieszkaBieńkowska-Szewczyk KrystynaSzewczyk B., Bergstrom Tomas. Human antibodies to herpes simplex virus type 1 glycoprotein C are neutralizing and target the heparan sulfate-binding domain. Virology 2010, 400(2): 197-206.

  47. Brzozowska AgnieszkaRychłowski MichałLipińska Andrea D.Bieńkowska-Szewczyk Krystyna. Point mutations in BHV-1 Us3 gene abolish its ability to induce cytoskeletal changes in various cell types. Veterinary Microbiology 2010, 143(1) Special Issue: 8-13.

  48. Dhillon Simrat, Witteveldt Jeroen, Gatherer Derek, Owsianka Ania M., Zeisel Mirjam B., Zahid Muhammad N., Rychłowska Małgorzata, Foung Steven K.H., Baumert Thomas F., Angus Allan G.N., Patel Arvind H. Mutations within a conserved region of the hepatitis C virus E2 glycoprotein that influence virus-receptor interactions and sensitivity to neutralizing antibodies. Journal of Virology 2010, 84(11): 5494-5507.

  49. Lipińska Andrea D.Bieńkowska-Szewczyk Krystyna. Nowe szczepionki przeciw herpeswirusom i wektory herpeswirusowe w terapii człowieka [Novel antiherpesviral vaccines and herpesviral vectors for human therapy]. Advances in Microbiology (Postępy Mikrobiologii) 2010, 49(3): 199-207.

  50. Morawska-Onyszczuk M.Bieńkowska-Szewczyk K., Dobbelstein M. Self-association of adenovirus type 5 E1B-55 kDa as well as p53 is essential for their mutual interaction. Oncogene 2010, 29(12): 1773-1786.

  51. Grabowska A. K., Lipińska A. D., Rohde J., Szewczyk B.Bieńkowska-Szewczyk K., Rziha H.-J. New baculovirus recombinants expressing Pseudorabies virus (PRV) glycoproteins protect mice against lethal challenge infection. Vaccine 2009, 27(27): 3584-3591.