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Laboratory of Protein Biochemistry | Intercollegiate Faculty of Biotechnology UG&MUG

Laboratory of Protein Biochemistry

Last modified: 
2019, November 29 - 11:39am

PROFILE

Research areas:

  • Chaperone proteins
  • Heat shock response
  • Mechanism of action of Hsp100 chaperones
  • Small heat shock proteins
  • Hsp70 and Hsp40 chaperones

RESEARCH TEAM

Laboratory of protein biochemistry

Department of Molecular and Cellular Biology
Intercollegiate Faculty of Biotechnology UG&MUG
Abrahama 58 Street, 80-307 Gdańsk, Poland

Office: Stanisława Urbańska
Tel.:+48 58 523 6421

team prof. Liberek

STAFF LIST

Head: Prof. Krzysztof Liberek [link]
tel.: +48 58 523 6346 [e-mail]

List of Employees and PhD students:

dr Agnieszka Kłosowska  [link]
tel.: +48 58 523 6359 [e-mail]
dr Szymon Ziętkiewicz  [link]
tel.: +48 58 523 6366 [e-mail]

Msc Tomasz Chamera
Msc Igor Obuchowski
Msc Dagmara Mróz
Msc Hubert Wyszkowski
Msc Piotr Karaś
Msc Wiktoria Sztangierska

tel.: +48 58 523 6359
 

PUBLICATION

  1. Obuchowski Igor, Piróg Artur, Stolarska Milena, Tomiczek Bartłomiej, Liberek Krzysztof. Duplicate divergence of two bacterial small heat shock proteins reduces the demand for Hsp70 in refolding of substrates. PLoS Genet  2019, 15(10): e1008479 (doi: 10.1371/journal.pgen.1008479)

  2. Chamera Tomasz, Kłosowska Agnieszka, Janta Anna, Wyszkowski Hubert, Obuchowski Igor, Gumowski Krzysztof, Liberek Krzysztof. Selective Hsp70-Dependent Docking of Hsp104 to Protein Aggregates Protects the Cell from the Toxicity of the Disaggregase. Journal of Molecular Biology 2019, 431(11): 2180-2196 (doi: 10.1016/j.jmb.2019.04.014)

  3. Carra Serena, Alberti Simon, Benesch Justin L. P., Boelens Wilbert, Buchner Johannes, Carver John A., Cecconi Ciro, Ecroyd Heath, Gusev Nikolai, Hightower Lawrence E., KlevitRachel E., Lee Hyun, Liberek Krzysztof, Lockwood Brent, Poletti Angelo, Timmerman Vincent, Toth Melinda E., Vierling Elizabeth, Wu Tangchun, Tanguay Robert M.. Small heat shock proteins: multifaceted proteins with important implications for life. Cell Stress & Chapereones 2019, 24 (2): 295-308 (doi: 10.1007/s12192-019-00979-z)

  4. Szymczak Marta, Ziętkiewicz Szymon, Kuncewicz Katarzyna, Rodziewicz-Motowidło Sylwia, Orlikowska Marta. Expression, purification, and efficient refolding of the extracellular domain of Escherichia coli-expressed signaling receptor herpesvirus entry mediator. Protein Expression and Purification 2019: 105450 (doi :10.1016/j.pep.2019.105450)

  5. Jakubiuk-Tomaszuk Anna, Pienkowski Victor Murcia, Ziętkiewicz Szymon, Rydzanicz Malorzata, Kosinska Joanna, Stawinski Piotr, Szuminski Michal, Ploski Rafal. Syndromic chorioretinal coloboma associated with heterozygous de novo RARA mutation affecting an amino acid critical for retinoic acid interaction. Clinical Genetics 2019, 96(4): 371-375 (DOI: 10.1111/cge.13611)

  6. Kampinga Harm H. Andreasson Claes, Barducci Alessandro, Cheetham Michael E., Cyr Douglas, Emanuelsson Cecilia, Genevaux Pierre, Gestwicki Jason E., Goloubinoff Pierre, Huerta-Cepas Jaime, Kirstein Janine, Liberek Krzysztof, Mayer Matthias P., Nagata Kazuhiro, Nillegoda Nadinath B.,Pulido Pablo; Ramos Carlos; De los Rios Paolo; Rospert Sabine, Rosenzweig Rina, Sahi Chandan, Taipale Mikko, Tomiczek Bratlomiej, Ushioda Ryo, Young Jason C., Zimmermann Richard, Zylicz Alicja, Zylicz Maciej, Craig Elizabeth A., Marszałek Jarosław. Function, evolution, and structure of J-domain proteins. Cell Stress & Chaperones 2019, 24(1): 7-15 (doi: 10.1007/s12192-018-0948-4)

  7. Lipska-Zietkiewicz Beata S, Gellermann Jutta, Boyer Olivia, Gribouval Olivier, Zietkiewicz Szymon, Kari Jameela A, Shalaby Mohamed A, Ozaltin Fatih, Dusek Jiri,  Melk Anette, Bayazit Aysun K, Massella Laura, Hyla-Klekot Lidia, Habbig Sandra, Godron Astrid, Szczepanska Maria, Bienias Beata, Drozdz Dorota, Odeh Rasha, Jarmuzek Wioletta, Zachwieja Katarzyna, Trautmann Agnes, Antignac Corinne, Schaefer Franz.  Low renal but high extrarenal phenotype variability in Schimke immuno-osseous dysplasia PlosOne 2017, 12(8) e0180926 (doi: 10.1371/journal.pone.0180926)       

  8. Carra Serena, Alberti Simon, Arrigo Patrick A., Benesch Justin L., Benjamin Ivor J., Boelens Wilbert, Bartelt-Kirbach Britta, Brundel Bianca J. J. M., Buchner Johannes, Bukau Bernd, Carver John A., Ecroyd Heath, Emanuelsson Cecilia, Finet Stephanie, Golenhofen Nikola, Goloubinoff  Pierre, Gusev Nikolai, Haslbeck Martin, Hightower Lawrence E., Kampinga Harm H., Klevit Rachel E., Liberek Krzysztof, Mchaourab Hassane S.,  McMenimen  Kathryn A., Poletti Angelo, Quinlan Roy, Strelkov Sergei V., Toth Melinda E., Vierling Elizabeth, Tanguay Robert M. The growing world of small heat shock proteins: from structure to functions. Cell Stress & Chaperons 2017 22(4): 601-611 (doi: 10.1007/s12192-017-0787-8

  9. Żwirowski SzymonKłosowska AgnieszkaObuchowski Igor, Nillegoda Nadinath B, Piróg ArturZiętkiewicz Szymon, Bukau Bernd, Mogk Axel, Liberek Krzysztof. Hsp70 displaces small heat shock proteins from aggregates to initiate protein refolding.  The EMBO Journal 2017, 36: 783-796 (doi: 10.15252/embj.201593378)

  10. Kłosowska Agnieszka, Chamera Tomasz, Liberek Krzysztof. Adenosine diphosphate restricts the protein remodeling activity of Hsp104 chaperone to Hsp70 assisted disaggregation. eLIFE 2016, (doi: 10.7554/eLife.1515)

  11. Wortmann Saskia B., Ziętkiewicz Szymon, Kousi Maria, Szklarczyk Radek, Haack Tobias B., Gersting Søren W., Muntau Ania C., Rakovic Aleksandar, Renkema G. Herma, Rodenburg Richard J., Strom Tim M., Meitinger Thomas, Rubio-Gozalbo M. Estela, Chruściel Elżbieta, Distelmaier Felix, Golzio Christelle, Jansen Joop H., van Karnebeek Clara, Lillquist Yolanda, Lücke Thomas, Õunap Katrin, Zordania Riina, Yaplito-Lee Joy, van Bokhoven Hans, Spelbrink Johannes N., Vaz Frédéric M., Pras-Raves Mia, Ploski Rafał, Pronicka Ewa, Klein Christine, Willemsen Michel A. A. P., de Brouwer Arjan P. M., Prokisch Holger, Katsanis Nicholas, Wevers Ron A. CLPB Mutations Cause 3-Methylglutaconic Aciduria, Progressive Brain Atrophy, Intellectual Disability, Congenital Neutropenia, Cataracts, Movement Disorder. American Journal of Human Genetics 2015, 96(2): 245-257 (doi: 10.1016/j.ajhg.2014.12.013).

  12. Czajkowski Robert, Ozymko Zofia, Żwirowski Szymon, Łojkowska Ewa. Complete genome sequence of a broad-host-range lytic Dickeya spp. bacteriophage φ{symbol}D5. Archives of Virology 2014, 159(11): 3153-3155 (doi: 10.1007/s00705-014-2170-8).

  13. Lenartowski Robert, Suwińska Anna, Prusińska Justyna, Gumowski Krzysztof, Lenartowska Marta. Molecular cloning and transcriptional activity of a new Petunia calreticulin gene involved in pistil transmitting tract maturation, progamic phase, and double fertilization. Planta 2014, 239(2): 696-703 (doi: 10.1007/s00425-013-1971-4).

  14. Lipińska NataliaZiętkiewicz SzymonSobczak AlicjaJurczyk AgnieszkaPotocki WojciechMorawiec EwaWawrzycka AleksandraGumowski Krzysztof, Ślusarz Magdalena, Rodziewicz-Motowidło Sylwia, Chruściel ElżbietaLiberek Krzysztof. Disruption of Ionic Interactions between the Nucleotide Binding Domain 1 (NBD1) and Middle (M) Domain in Hsp100 Disaggregase Unleashes Toxic Hyperactivity and Partial Independence from Hsp70. Journal of Biological Chemistry 2013, 288(4): 2857-2869

  15. Nowicki Łukasz, Leźnicki Paweł, Morawiec EwaLitwińczuk NataliaLiberek Krzysztof. Role of a conserved aspartic acid in nucleotide binding domain 1 (NBD1) of Hsp100 chaperones in their activities. Cell stress & chaperones 2012, 17(3): 361-373

  16. Stróżecka JoannaChruściel Elzbieta, Górna Emilia, Szymańska Aneta, Ziętkiewicz SzymonLiberek Krzysztof. Importance of N- and C-terminal Regions of IbpA, Escherichia coli small heat shock protein, for chaperone function and oligomerization. Journal of Biological Chemistry 2012, 287(4): 2843-2853

  17. Ratajczak ElżbietaStróżecka Joanna, Matuszewska Marlena, Ziętkiewicz Szymon, Kuczyńska-Wiśnik Dorota, Laskowska Ewa, Liberek Krzysztof. IbpA the small heat shock protein from Escherichia coli forms fibrils in the absence of its cochaperone IbpB. FEBS Letters 2010, 584(11): 2253-2257

  18. Ziętkiewicz SzymonLiberek Krzysztof. Dispose to the pole-protein aggregation control in bacteria. EMBO Journal 2010, 29(5): 869-870

  19. Ziętkiewicz Szymon, Ślusarz Magdalena J., Ślusarz Rafał, Liberek Krzysztof, Rodziewicz-Motowidło Sylwia. Conformational stability of the full-atom hexameric model of the ClpB chaperone from Escherichia coliBiopolymers 2010, 93(1): 47-60

  20. Lipska B.S., Drożyńska E., Scaruffi P., Tonini G.P., Iżycka-Świeszewska E., Ziętkiewicz S., Balcerska A., Perek D., Chybicka A., Biernat W., Limon J. c.1810C > T Polymorphism of NTRK1 Gene is associated with reduced Survival in Neuroblastoma Patients. BMC Cancer 2009, 9: art no: 439

  21. Matuszewska E., Kwiatkowska J., Ratajczak E., Kuczyńska-Wiśnik D., Laskowska E. Non-reducing sugar levels in beech (Fagus sylvatica) seeds as related to withstanding desiccation and storage. Acta Biochimica Polonica2009, 56(1): 55-61

  22. Ratajczak E.Ziętkiewicz S.Liberek K. Distinct activities of Escherichia coli small heat shock proteins IbpA and IbpB promote efficient protein disaggregation. Journal of Molecular Biology 2009, 386(1): 178-189