Laboratory of Biophysics

Laboratory of Biophysics deals with studying various nanoparticles and biologically active low molecular weight compounds, including anti-cancer drugs, antibiotics, environmental mutagens and carcinogens, toxins, and substances with chemopreventive properties. Fullerene C₆₀ is an example of a carbon nanoparticle that is highly hydrophobic and has significant antioxidant potential. The development of the aqueous solution of fullerene C₆₀ (FAS) allowed for its safe use in biological systems. Therefore, we are investigating the potential properties of FAS in modulating the activity of anticancer drugs. Additionally, together with our colleagues from external laboratories, we use the antioxidant properties of FAS in research on muscle fatigue and damage. Moreover, metallic nanoparticles (e.g. silver, platinum, or iron) have become the subject of research in our Laboratory as potential carriers of anticancer drugs and modulators of their activity. The main goal of these studies is to develop optimal systems for anticancer drugs transport and action modulation, which may lead to an increase in their effectiveness against cancer cells, while reducing their side effects. In our daily work, we use a wide range of physicochemical, biophysical, biological, and computational methods.

Biomolecular Systems Simulation subgroup (SUB), whose leader is Prof. Rajmund Kaźmierkiewicz, is a part of the Biophysics Laboratory. The tools used in the SUB research activities are all contemporary molecular modeling techniques including, but not limited to, quantum ab-initio methods, semi-empirical methods, empirical – molecular mechanics (classical force fields methods), molecular dynamics simulations, Monte-Carlo methods, calculations taking into consideration the free energy changes and the influence of solvent. The computers used in research include five supercomputers placed near the beginning of the top500 list including the second fastest computer in the EU which is IBM Blue Gene located in Forschungszentrum Juelich, Germany. Each of our computer modeling projects takes into consideration the complete environment with the presence of all molecules, as they appear in the living organisms.

Laboratory of Biophysics
Intercollegiate Faculty of Biotechnology UG&MUG
Abrahama 58 Street, 80-307 Gdańsk, Poland

tel.: +48 58 523 6311

STAFF LIST

Head: Assoc. Prof. Jacek Piosik [link]
tel.: +48 58 523 63 11 [e-mail] 

List of PhD and PhD students:

Assoc. Prof. Rajmund Kaźmierkiewicz
tel.: +48 58 523 6340 [e-mail] 

PhD Grzegorz Gołuński [link]
tel: +48 58 523 6310 [email] 

MSc Patrycja Bełdzińska
tel: 58 523 6310 [email], p. 268 AB
MSc Marcin Zakrzewski
tel: 58 523 6310 [email], p. 268 AB
MSc Inga Jamrożek
tel.: +48 58 523 6341, p. 302
MSc Monika Romanik-Błońska
tel.: +48 58 523 6341, p. 302

1. Motuziuk Olexandr, Nozdrenko Dmytro, Prylutska Svitlana, Vareniuk Igor, Bogutska Kateryna, Braniuk Serhii, Korotkyi Olexandr, Prylutskyy Yuriy, Ritter Uwe, Piosik Jacek. The effect of C60 fullerene on the mechanokinetics of muscle gastrocnemius contraction in chronically alcoholized rats. Heliyon 2023, 9(8): e18745 (doi: 10.1016/j.heliyon.2023.e18745)

2. Han Xuexiang, Alameh Mohamad-Gabriel, Butowska Kamila, Knox James J. , Lundgreen Kendall , Ghattas Majed , Gong Ningqiang, Xue Lulu, Xu Ying , Lavertu Marc, Bates Paul, Xu Junchao, Nie Guangjun, Zhong Yi, Weissman Drew, Mitchell Michael J.. Adjuvant lipidoid-substituted lipid nanoparticles augment the immunogenicity of SARS-CoV-2 mRNA vaccines. Nature Nanotechnology 2023, 18(9): 1105-1114 (doi: 10.1038/s41565-023-01404-4)

3. Zavodovskiy Danylo O., Bulgakova Nataliya V., Sokolowska Inna, Prylutskyy Yuriy I., Ritter Uwe, Gonchar Olga O., Kostyukov Alexander I., Vlasenko Oleh V., Butowska Kamila, Borowik Agnieszka, Piosik Jacek, Maznychenko Andriy. Water-soluble pristine C60 fullerenes attenuate isometric muscle force reduction in a rat acute inflammatory pain model. BMC Musculoskeletal Disorders 2023, 24: 606 (doi: 10.1186/s12891-023-06719-w)

4. Butowska Kamila, Han Xuexiang, Gong Ningqiang, El-Mayta Rakan, Haley Rebecca M., Xue Lulu, Zhong Wenqun, Guo Wei, Wang Karin, Mitchell Michael J.. Doxorubicin-conjugated siRNA lipid nanoparticles for combination cancer therapy. Acta Pharmaceutica Sinica B 2023, 13(4): 1429-1437 (doi: 10.1016/j.apsb.2022.07.011)

5. Nozdrenko Dmytro, Prylutska Svitlana, Bogutska Kateryna, Cherepanov Vsevolod, Senenko Anton, Vygovska Oksana, Khrapatyi Sergii, Ritter Uwe, Prylutskyy Yuriy, Piosik Jacek. Analysis of Biomechanical and Biochemical Markers of Rat Muscle Soleus Fatigue Processes Development during Long-Term Use of C60 Fullerene and N-acetylcysteine. Nanomaterials 2022, 12: 1552 (doi: 10.3390/nano12091552)

6. Woziwodzka Anna, Krychowiak-Maśnicka Marta, Gołuński Grzegorz, Łosiewska Anna, Borowik Agnieszka, Wyrzykowski Dariusz, Piosik Jacek. New Life of an Old Drug: Caffeine as a Modulator of Antibacterial Activity of Commonly Used Antibiotics. Pharmaceuticals 2022, 15(7): 872 (doi: 10.3390/ph15070872)

7. Sikora Karol, Nowacki Andrzej, Szweda Piotr, Woziwodzka Anna, Bartoszewska Sylwia, Piosik Jacek, Dmochowska Barbara. Antimicrobial, cytotoxic and mutagenic activity of gemini QAS derivatives of 1,4:3,6-dianhydro-L-iditol. Molecules 2022, 27(3): 1-13 (doi: 10.3390/molecules27030757)

8. Zhang Hanwen, Han Xuexiang, Alameh Mohamad-Gabriel, Shepherd Sarah J., Padilla Marshall S., Xue Lulu, Butowska Kamila, Weissman Drew, Mitchell Michael J.. Rational design of anti-inflammatory lipid nanoparticles for mRNA delivery. Journal of Biomedical Materials Research A 2022, 110(5): 1101-1108 (doi: 10.1002/jbm.a.37356)

9. Han Xuexiang, Zhang Hanwen, Butowska Kamila, Swingle Kelsey L., Alameh Mohamad-Gabriel, Weissman Drew, Mitchell Michael J.. An ionizable lipid toolbox for RNA delivery. Nature Communication 2021, 12: 7233 (doi: 10.1038/s41467-021-27493-0)

10. Horak Iryna, Prylutska Svitlana, Krysiuk Iryna, Luhovskyi Serhii, Hrabovsky Oleksii, Tverdokhleb  Nina, Franskevych Daria, Rumiantsev Dmytro, Senenko Anton, Evstigneev Maxim, Drobot Liudmyla, Matyshevska Olga, Ritter Uwe, Piosik Jacek, Prylutskyy Yuriy. Nanocomplex of Berberine with C60 Fullerene Is a Potent Suppressor of Lewis Lung Carcinoma Cells Invasion In Vitro and Metastatic Activity In Vivo. Materials 2021, 14: 6114 (doi: 10.3390/ma14206114)

11. Chernykh Mariia, Zavalny Dmytro, Sokolova Viktoriya, Ponomarenko Stanislav, Prylutska Svitlana, Kuziv Yuliia, Chumachenko Vasyl, Marynin Andrii, Kutsevol Nataliya, Epple Matthias, Ritter Uwe, Piosik Jacek, Prylutskyy Yuriy. A New Water-Soluble Thermosensitive Star-Like Copolymer as a Promising Carrier of the Chemotherapeutic Drug Doxorubicin. Materials 2021, 14(13): 3517 (doi: 10.3390/ma14133517)

12. Woziwodzka Anna, Krychowiak-Maśnicka Marta, Gołuński Grzegorz, Felberg Anna, Borowik Agnieszka, Wyrzykowski Dariusz, Piosik Jacek. Modulatory Effects of Caffeine and Pentoxifylline on Aromatic Antibiotics: A Role for Hetero-Complex Formation. Molecules 2021, 26: 3628 (doi: 10.3390/molecules26123628)

13. Gonchar Olga O., Maznychenko Andriy V., Klyuchko Olena M., Mankovska Iryna M., Butowska Kamila, Borowik Agnieszka, Piosik Jacek, Sokolowska Inna. C60 Fullerene Reduces 3-Nitropropionic Acid-Induced Oxidative Stress Disorders and Mitochondrial Dysfunction in Rats by Modulation of p53, Bcl-2 and Nrf2 Targeted Proteins. International Journal of Molecular Sciences 2021, 22: 5444 (doi: 10.3390/ijms22115444)

14. Butowska Kamila, Woziwodzka Anna, Borowik Agnieszka, Piosik Jacek. Polymeric Nanocarriers: A Transformation in Doxorubicin Therapies. Materials 2021, 14(9): 2135 (doi: 10.3390/ma14092135)

15. Butowska Kamila, Żamojć Krzysztof, Kogut Mateusz, Kozak Witold, Wyrzykowski Dariusz, Wiczk Wiesław, Czub Jacek, Piosik Jacek, Rak Janusz. The Product of Matrix Metalloproteinase Cleavage of Doxorubicin Conjugate for Anticancer Drug Delivery: Calorimetric, Spectroscopic, and Molecular Dynamics Studies on Peptide–Doxorubicin Binding to DNA. International Journal o f Molecular Sciences 2020, 21, 6923 (doi:10.3390/ijms21186923)

16. Maznychenko Andriy V., Bulgakova Nataliya V., Sokolowska Inna V., Butowska Kamila, Borowik Agnieszka, Mankivska Olena P., Piosik Jacek, Tomiak Tomasz, Gonchar Olga O., Maisky Volodymyr O., Kostyukov  Alexander I. . Fatigue-induced Fos immunoreactivity within the lumbar cord and amygdala decreases after С60 fullerene pretreatment. Scientific Reports 2020, 10: 9826 (doi: 10.1038/s41598-020-67034-1)

17. Myszka Henryk, Grzywacz Daria, Zdrowowicz Magdalena, Spisz Paulina, Butowska Kamila, Rak Janusz, Piosik Jacek, Jaśkiewicz Maciej, Kamysz Wojciech, Liberek Beata. Design, synthesis and biological evaluation of betulin-3-yl 2-amino-2-deoxy- β-D-glycopyranosides. Bioorganic Chemistry 2020, 96: 103568 (doi: 10.1016/j.bioorg.2020.103568)

18. Woziwodzka Anna, Rybicka Magda, Sznarkowska Alicja, Romanowski Tomasz, Dreczewski Marcin, Stalke Piotr, Bielawski Krzysztof Piotr. TNF-alpha polymorphisms affect persistence and progression of HBV infection. Molecular Genetics & Genome Medicine 2019, 7(10): UNSP e935  (doi: 10.1002/mgg3.935)

19. Butowska Kamila, Kozak Witold, Zdrowowicz Magdalena, Makurat Samanta, Rychłowski Michał, Hać Aleksandra, Herman-Antosiewicz Anna, Piosik Jacek, Rak Janusz. Cytotoxicity of doxorubicin conjugated with C60 fullerene. Structural and in vitro studies. Structural Chemistry 2019, 30(6): 2327-2338 (doi: 10.1007/s11224-019-01428-4)

20. Rapacka-Zdończyk Aleksandra, Woźniak Agata, Pierański Michał, Woziwodzka Anna, Bielawski Krzysztof P., Grinholc Mariusz.Development of Staphylococcus aureus tolerance to antimicrobial photodynamic inactivation and antimicrobial blue light upon sub-lethal treatment. Scientific Reports 2019, 9:  9423  (doi: 10.1038/s41598-019-45962-x)

21. Borowik Agnieszka, Butowska Kamila, Konkel Kinga, Banasiuk Rafał, Derewońko Natali, Wyrzykowski Dariusz, Davydenko Mykola, Cherepanov Vsevolod, Styopkin Viktor, Prylutskyy Yuriy, Pohl Paweł, Królicka Aleksandra, Piosik Jacek. The impact of surface functionalization on the biophysical properties of silver nanoparticles. Nanomaterials 2019, 9: 973 (doi: 10.3390/nano9070973)

22. Borowik Agnieszka, Banasiuk Rafał, Derewonko Natalia, Rychłowski Michał, Krychowiak-Maśnicka Marta, Wyrzykowski Dariusz, Ziąbka Magdalena, Woziwodzka Anna, Królicka Aleksandra, Piosik Jacek. Interactions of newly synthesized platinum nanoparticles with ICR-191 and their potential application. Scientific Reports 2019, 9: 4987 (doi: 10.1038/s41598-019-41092-6)

23. Ameixa J., Arthur-Baidoo E., Meißner R., Makurat S., Kozak W., Butowska Kamila, Ferreira da Silva F., Rak J., Denifl S.. Low-energy electron-induced decomposition of 5-trifluoromethanesulfonyl-uracil: A potential radiosensitizer. Journal Of Chemical Physics 2018, 149: 16 (doi: 10.1063/1.5050594)

24. Krychowiak Marta, Kawiak Anna, Narajczyk Magdalena, Borowik Agnieszka, Królicka Aleksandra. Silver Nanoparticles Combined With Naphthoquinones as an Effective Synergistic Strategy Against Staphylococcus aureus. Frontiers in Pharmacology 2018, 9: 816 (doi: 10.3389/fphar.2018.00816)

25. Gołuński Grzegorz, Woziwodzka Anna, Piosik Jacek. Potential use of pentoxifylline in cancer therapy. Current Pharmaceutical Biotechnology 2018, 19(3): 206-216 (doi: 10.2174/1389201019666180528084641)

26. Agnieszka Borowik, Prylutskyy Yuriy, Kawelski Łukasz, Kyzyma Olena, Bulavin Leonid, Ivankov Oleksandr, Cherepanov Vsevolod, Wyrzykowski Dariusz, Kaźmierkiewicz Rajmund, Gołuński Grzegorz, Woziwodzka Anna, Evstigneev Maxim, Ritter Uwe, Jacek Piosik. Does C60fullerene act as a transporter of small aromatic molecules? Colloids and Surfaces B: Biointerfaces 2018, 164: 134-143 (doi: 10.1016/j.colsurfb.2018.01.026)

27. Grela Anna, Jamrożek Inga, Hubisz Marta, Iwanicki Adam, Hinc Krzysztof, Kaźmierkiewicz Rajmund, Obuchowski Michał. Positions 299 and 302 of the GerAA subunit are important for function of the GerA spore germination receptor in Bacillus subtilis. PLOS One 2018, 13(6); e0198561 (doi: 10.1371/journal.pone.0198561)

28. Agnieszka Borowik, Prylutskyy Yuriy, Kawelski Łukasz, Kyzyma Olena, Bulavin Leonid, Ivankov Oleksandr, Cherepanov Vsevolod, Wyrzykowski Dariusz, Kaźmierkiewicz Rajmund, Gołuński Grzegorz, Woziwodzka Anna, Evstigneev Maxim, Ritter Uwe,Jacek Piosik. Does C60fullerene act as a transporter of small aromatic molecules? Colloids and Surfaces B: Biointerfaces 2018, 164: 134-143 (doi: 10.1016/j.colsurfb.2018.01.026)

29. Prylutska Svitlana, Panchuk Rostyslav, Gołuński Grzegorz, Skivka Larysa, Prylutskyy Yuriy, Hurmach  Vasyl, Skorohyd Nadya, Borowik Agnieszka , Woziwodzka Anna, Piosik Jacek, Kyzyma Olena, Garamus Vasil, Bulavin Leonid, Evstigneev Maxim, Buchelnikov Anatoly, Stoika Rostyslav, Berger Walter, Ritter Uwe, Scharff Peter. C60 fullerene enhances cisplatin anticancer activity and overcomes tumor cells drug resistance. Nano Research 2017, 10(2): 652–671 (doi: 10.1007/s12274-016-1324-2)

30. Banecka-Majkutewicz Zyta, Kadzinski  Leszek, Grabowski  Michał, Bloch Sylwia, Kazmierkiewicz Rajmund, Jakobkiewicz-Banecka Joanna, Gabig-Ciminska Magdalena, Wegrzyn Grzegorz, Wegrzyn Alicja, Banecki Bogdan. Evidence for interactions between homocysteine and genistein: insights into stroke risk and potential treatment.  Metabolic Brain Disease 2017,  32(6) : 1855-1860 (doi: 10.1007/s11011-017-0078-1)

31. Wróblewska Anna, Bernat Agnieszka, Woziwodzka Anna, Markiewicz Joanna, Romanowski Tomasz, Bielawski Krzysztof P, Smiatacz Tomasz, Sikorska Katarzyna. Interferon lambda polymorphisms associate with body iron indices and hepatic expression of interferon-responsive long non-coding RNA in chronic hepatitis C. Clinical and Experimental Medicine 2016, (doi: 10.1007/s10238-016-0423-4)

32. Dmochowska Barbara, Sikora Karol, Woziwodzka Anna, Piosik Jacek, Podgórska Beata. Mutagenic activity of quaternary ammonium salt derivatives of carbohydrates. Beilstein Journal of Organic Chemistry 2016, 12: 1434-1439 (doi: 10.3762/bjoc.12.138)

33. Gołuński Grzegorz, Borowik Agnieszka, Derewońko Natalia, Kawiak Anna, Rychłowski Michał, Woziwodzka Anna, Piosik Jacek . Pentoxifylline as a modulator of anticancer drug doxorubicin. Part II: Reduction of doxorubicin DNA binding and alleviation of its biological effects. Biochimie 2016, 123: 95-102 (doi: 10.1016/j.biochi.2016.02.003).

34. Gołuński Grzegorz, Borowik Agnieszka, Lipińska Andrea, Romanik Monika, Derewońko Natalia, Woziwodzka Anna, Piosik Jacek. Pentoxifylline affects idarubicin binding to DNA. Bioorganic Chemistry 2016, 65: 118-125 (doi: 10.1016/j.bioorg.2016.02.005).

35. Prylutskyy Y., Borowik A., Gołuński G., Woziwodzka A., Piosik J., Kozyma O., Pashkova I., Ritter U., Evstigneev M. Biophysical characterization of the complexation of C60 fullerene with doxorubicin in a prokaryotic model. Materials Science & Engineering Technology (Materialwissenschaft und Werkstofftechnik) 2016, 47(2-3): 92-97 (doi: 10.1002/mawe.201600463).

36. Makarewicz Tomasz, Kaźmierkiewicz Rajmund. Improvements in GROMACS plugin for PyMOL including implicit solvent simulations and displaying results of PCA analysis. Journal of Molecular Modeling 2016, 22: 109 (doi: 10.1007/s00894-016-2982-4).

37. Kossakowska-Zwierucho Monika, Kaźmierkiewicz Rajmund, Bielawski Krzysztof P., Nakonieczna Joanna. Factors determining staphylococcus aureus susceptibility to photoantimicrobial chemotherapy: RsbU activity, ataphyloxanthin level, and membrane fluidity. Frontiers in Microbiology 2016, 7: 1141 (doi: 10.3389/fmicb.2016.01141).

38. Gołuński Grzegorz, Borowik Agnieszka, Wyrzykowski Dariusz, Woziwodzka Anna, Piosik Jacek. Pentoxifylline as a modulator of anticancer drug doxorubicin. Part I: reduction of doxorubicin DNA binding. Chemico-Biological Interactions 2015, 242: 291-298 (doi: 10.1016/j.cbi.2015.10.008).

39. Prylutskyy Yu. I., Cherepanov V. V., Evstigneev M. P., Kuzyma O. A., Petrenko V. I., Styopkin V. I., Bulavin L. A., Davidenko N. A., Wyrzykowski D., Woziwodzka A., Piosik J., Kaźmierkiewicz R., Ritter U. Structural self-organization of C60 and cisplatin in physiological solution. Physical Chemistry Chemical Physics 2015,17(39): 26084-26092 (doi: 10.1039/c5cp02688a).

40. Śledź Wojciech, Łoś Emilia, Pączek Agnieszka, Rischka Jacek, Motyka Agata, Żołędowska Sabina, Piosik Jacek, Łojkowska Ewa. Antibacterial activity of caffeine against plant pathogenic bacteria. Acta Biochimica Polonica 2015, 62(3): 605-612 (doi: 10.18388/abp.2015_1092).

41. Grabowski Michał, Banecki Bogdan, Kadziński Leszek, Jakóbkiewicz-Banecka Joanna, Kaźmierkiewicz Rajmund, Gabig-Cimińska Magdalena, Węgrzyn Grzegorz, Węgrzyn Alicja, Banecka-Majkutewicz Zyta. Genistein inhibits activities of methylenetetrahydrofolate reductase and lactate dehydrogenase, enzymes which use NADH as a substrate. Biochemical and Biophysical Research Communications 2015, 465(3): 363-367 (doi: 10.1016/j.bbrc.2015.08.004).

42. Pikora Mateusz, Giełdoń Artur. RASMOL AB - New functionalities in the program for structure analysis. Acta Biochimica Polonica 2015, 62(3): 629-631 (doi: 10.18388/abp.2015_972).

43. Prylutskyy Yu. I., Cherepanov V. V., Evstigneev M. P., Kuzyma O. A., Petrenko V. I., Styopkin V. I., Bulavin L. A., Davidenko N. A., Wyrzykowski D., Woziwodzka A., Piosik J., Kaźmierkiewicz R., Ritter U. Structural self-organization of C60 and cisplatin in physiological solution. Physical Chemistry Chemical Physics 2015, 17(39): 26084-26092 (doi: 10.1039/c5cp02688a).

44. Barabaś Anna, Piosik Jacek, Gołuński Grzegorz, Kawecka Alicja, Chylewska Agnieszka, Chmurzyński Lech, Dąbrowska Aleksandra. Ocena aktywności biologicznej serii 3-azydowych izomerów monosacharydów. Na pograniczu chemii i biologii. Red. Henryk Koroniak, Jan Barciszewski. Wydawnictwo Naukowe Uniwersytetu im. Adama Mickiewicza, Poznań 2014, T. 33: 177-184 (ISBN: 978-83-232-2803-5)

45. Prylutskyy Yu. I., Evstigneev M. P., Pashkova I. S., Wyrzykowski D., Woziwodzka A., Gołuński G., Piosik J., Cherepanov V. V., Ritter U. Characterization of C₆₀ fullerene complexation with antibiotic Doxorubicin. Physical Chemistry Chemical Physics 2014, 16(42): 23164-23172 (doi: 10.1039/C4CP03367A).

46. Voronin Dmitry P., Buchelnikov Anatoly S., Kostjukov Viktor V., Khrapatiy Sergii V., Wyrzykowski Dariusz, Piosik J., Prylutskyy Yuriy I., Ritter Uwe, Evstigneev Maxim P. Evidence of entropically-driven C₆₀ fullerene aggregation in aqueous solution. Journal of Chemical Physics 2014, 140: art. no 104909 (1-5) (doi: 10.1063/1.4867902).

47. Platt Randall J., Curtice Kigen J., Twede Vernon D.,Watkins Maren, Gruszczyński Paweł, Bulaj Grzegorz, Horvath Martin P., Olivera Baldomero M.. From molecular phylogeny towards differentiating pharmacology for NMDA receptor subtypes. Toxicon 2014, 81: 67-79 (doi: 10.1016/j.toxicon.2014.01.016).

48. Dmochowska Barbara, Sikora Karol, Woziwodzka Anna, Piosik Jacek, Wiśniewski Andrzej. Właściwości mutagenne czwartorzędowych soli alditolilo- i glikozylo-amoniowych [Mutagenic activities of quaternary alditolyl- and glucosylammonium salts]. Przemysł Chemiczny 2013, 92(9): 1585-1588.

49. Gołuński Grzegorz, Woziwodzka Anna, Iermak Ievgeniia, Rychłowski Michał, Piosik Jacek. Modulation of acridine mutagen ICR191 intercalation to DNA by methylxanthines - analysis with mathematical models. Bioorganic & Medicinal Chemistry 2013, 21(11): 3280-3289 (doi: 10.1016/j.bmc.2013.03.043).

50. Woziwodzka Anna, Gołuński Grzegorz, Piosik Jacek. Heterocyclic aromatic amines hetero-complexation with biologically-active aromatic compounds and its possible role in chemoprevention. ISRN Biophysics 2013, 2013: art. no 740821 (1-11) (doi: 10.1155/2013/740821).

51. Woziwodzka Anna, Gołuński Grzegorz, Wyrzykowski Dariusz, Kaźmierkiewicz Rajmund, Piosik Jacek. Caffeine and other methylxanthines as interceptors of food-borne aromatic mutagens: inhibition of Trp-P-1 and Trp-P-2 mutagenic activity. Chemical Research in Toxicology 2013, 26(11): 1660−1673 (doi: 10.1021/tx4002513).

52. Jędrzejewski Roch P., Kaźmierkiewicz Rajmund. Structure of Patt1 human proapoptotic histone acetyltransferase. Journal of Molecular Modeling 2013, 19(12): 5533-5538.

53. Makarewicz Tomasz, Kaźmierkiewicz Rajmund. Molecular Dynamics Simulation by GROMACS Using GUI Plugin for PyMOL. Journal of Chemical Information and Modeling 2013, 53(5): 1229-1234.

54. Woziwodzka Anna, Gołuński Grzegorz, Wyrzykowski Dariusz, Kaźmierkiewicz Rajmund, Piosik Jacek. Caffeine and other methylxanthines as interceptors of food-borne aromatic mutagens: inhibition of Trp-P-1 and Trp-P-2 mutagenic activity. Chemical Research in Toxicology 2013, 26(11): 1660-1673.

55. Śledź Wojciech, Adamowska Aleksandra, Piosik Jacek, Łojkowska Ewa. Detecting live and dead cells of Pectobacterium atrosepticum based on immunomagnetics separation and staining. Journal of Plant Pathology 2012, 94(3): 681-685 (doi: 10.4454/JPP.FA.2012.050).

56. Platt Randall J., Han Tiffany S., Green Brad R., Smith Misty D., Skalicky Jack, Gruszczyński Paweł, White H. Steve, Olivera Baldomero, Bulaj Grzegorz, Gajewiak Joanna. Stapling Mimics Noncovalent Interactions of gamma-Carboxyglutamates in Conantokins, Peptidic Antagonists of N-Methyl-D-Aspartic Acid Receptors. Journal of Biological Chemistry 2012, 287(24): 20727-20736.

57. Dmochowska Barbara, Piosik Jacek, Woziwodzka Anna, Sikora Karol, Wiśniewski Andrzej, Węgrzyn Grzegorz. Mutagenicity of quaternary ammonium salts containing carbohydrate moieties. Journal of Hazardous Materials 2011, 193(1): 272-278 (doi: 10.1016/j.jhazmat.2011.07.064).

58. Sikora Karol, Dmochowska Barbara, Woziwodzka Anna, Piosik Jacek, Węgrzyn Grzegorz, Wiśniewski Andrzej. O bromkach D-glikopiranozyd 2-amonioetylu. W: Na pograniczu chemii i biologii. Red. Henryk Koroniak, Jan Barciszewski. Wydawnictwo Naukowe Uniwersytetu im. Adama Mickiewicza w Poznaniu (ISBN 978-83-232-2368-9), Poznań 2011, T. XXVII: 55-65.

59. Woziwodzka Anna, Gwizdek-Wiśniewska Anna, Piosik Jacek. Caffeine, pentoxifylline and theophylline form stacking complexes with IQ-type heterocyclic aromatic amines. Bioorganic Chemistry 2011, 39(1): 10-17 (doi: 10.1016/j.bioorg.2010.11.001).

60. Gruszczyński P., Smalara K., Obuchowski M., Kaźmierkiewicz R. ATP and its N6-substituted analogues: parameterization, molecular dynamics simulation and conformational analysis. Journal of Molecular Modeling 2011, 17(5): 1081-1090.

61. Piosik Jacek, Wasielewski Kacper, Woziwodzka Anna, Śledź Wojciech, Gwizdek-Wiśniewska Anna. De-intercalation of ethidium bromide and propidium iodine from DNA in the presence of caffeine. Central European Journal of Biology 2010, 5(1): 59-66 (doi: 10.2478/s11535-009-0077-2).

62. Woziwodzka Anna, Tarasewicz Marta, Piosik Jacek. Metabolizm heterocyklicznych amin aromatycznych - mutagenów/kancerogenów izolowanych z żywności [Heterocyclic aromatic amines, food-derived mutagens: metabolism and relevance to cancer susceptibility]. Postępy Biochemii 2010, 56(4): 435-446.

63. Gruszczyński Paweł, Obuchowski Michał, Kaźmierkiewicz Rajmund. Phosphorylation and ATP-binding induced conformational changes in the PrkC, Ser/Thr kinase from B. subtilis. Journal of Computer-Aided Molecular Design 2010, 24(9): 733-747.

64. Zhang Min-Min, Gruszczyński Paweł, Walewska Aleksandra, Bulaj Grzegorz, Olivera Baldomero M., Yoshikami Doju. Cooccupancy of the outer vestibule of voltage-gated sodium channels by μ-conotoxin KIIIA and saxitoxin or tetrodotoxin. Journal of Neurophysiology 2010, 104(1): 88-97.

65. Woziwodzka Anna, Piosik Jacek Heterocykliczne aminy aromatyczne: charakterystyka i znaczenie w indukcji procesów nowotworowych [Heterocyclic aromatic amines and their role in the induction of carcinogenesis]. Biotechnologia 2009, 4(87): 133-151 (pdf).

66. Florek A., Kaźmierkiewicz R. Modelowanie molekularne - przegląd zastosowań w naukach przyrodniczych [Molecula modelling - what does it exactly mean?]. W: Research, theory, and practice in chemistry didactics XIX, Part 2, Wydawnictwo Uniwersytet Hradec Kralove 2009 (ISBN 978-80-7041-839-0) 297-305.

67. Han T.S., Zhang M.-M., Walewska A., Gruszczyński P., Robertson C.R., Cheatham III T.E., Yoshikami D., Olivera B.M., Bulaj G. Structurally minimized μ-conotoxin analogues as sodium channel blockers: implications for designing conopeptide-based therapeutics. ChemMedChem 2009, 4(3): 406-414.

68. Twede V.D., Teichert R.W., Walker C.S., Gruszczyński P., Kaźmierkiewicz R., Bulaj G., Olivera B.M. Conantokin-Br from Conus brettinghami and selectivity determinants for the NR2D subunit of the NMDA receptor. Biochemistry 2009, 48(19): 4063-4073.

69. Kawiak A., Piosik J., Stasiłojć G., Gwizdek-Wiśniewska A., Marczak L., Stobiecki M., Bigda J., Łojkowska E. Induction of apoptosis by plumbagin through reactive oxygen species-mediated inhibition of topoisomerase II. Toxicology and Applied Pharmacology 2007, 223(3): 267-276 (doi: 10.1016/j.taap.2007.05.018).

70. Ulanowska Katarzyna, Piosik Jacek, Gwizdek-Wiśniewska Anna, Węgrzyn Grzegorz. Impaired mutagenic activities of MPDP+ (1-methyl-4-phenyl-2,3-dihydropyridinium) and MPP+ (1-methyl-4-phenylpyridinium) due to their interactions with methylxanthines. Bioorganic & Medicinal Chemistry 2007, 15(15): 5150-5157 (doi: 10.1016/j.bmc.2007.05.025).

71. Piosik Jacek, Gwizdek-Wiśniewska Anna, Ulanowska Katarzyna, Ochociński Jakub, Czyż Agata, Węgrzyn Grzegorz. Methylxanthines (caffeine, pentoxifylline and theophylline) decrease the mutagenic effect of daunomycin, doxorubicin and mitoxantrone. Acta Biochimica Polonica 2005, 52(4): 923-926 (pdf).

72. Ulanowska Katarzyna, Piosik Jacek, Gwizdek-Wiśniewska Anna, Węgrzyn Grzegorz. Formation of stacking complexes between caffeine (1,2,3-trimethylxanthine) and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine may attenuate biological effects of this neurotoxin. Bioorganic Chemistry 2005, 33: 402-413 (doi: 10.1016/j.bioorg.2005.07.004).

73. Piosik Jacek, Ulanowska Katarzyna, Gwizdek-Wiśniewska Anna, Czyż Agata, Kapuściński Jan, Węgrzyn Grzegorz. Alleviation of mutagenic effects of polycyclic aromatic agents (quinacrine mustard, ICR-191 and ICR-170) by caffeine and pentoxifylline. Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis 2003, 530(1-2): 47-57 (doi: 10.1016/S0027-5107(03)00136-2).

74. Kapuściński J., Ardelt B., Piosik J., Zdunek M., Darzynkiewicz Z. The modulation of the DNA-damaging effect of polycyclic aromatic agents by xanthines. Part I. Reduction of cytostatic effects of quinacrine mustard by caffeine. Biochemical Pharmacology 2002, 63(4): 625-634 (doi: 10.1016/S0006-2952(01)00904-2).

75. Piosik Jacek, Zdunek Małgorzata, Kapuściński Jan. The modulation by xanthines of the DNA-damaging effect of polycyclic aromatic agents. Part II. The stacking complexes of caffeine with doxorubicin and mitoxantrone. Biochemical Pharmacology 2002, 63(4): 635-646 (doi: 10.1016/S0006-2952(01)00903-0).

76. Zdunek Małgorzata, Piosik Jacek, Kapuściński Jan. Thermodynamical model of mixed aggregation of ligands with caffeine in aqueous solution. Part II. Biophysical Chemistry 2000, 84(1): 77-85 (doi: 10.1016/S0301-4622(99)00152-0).

77. Piechula Stanisław, Piosik Jacek, Bielawski Krzysztof P., Podhajska Anna J. Isolation and characterization of the restriction endonuclease Ppel from Phormidium persicinum. Molecular Biotechnology 1996, 5(2): 97-99 (doi: 10.1007/BF02789059).