Conferences | Intercollegiate Faculty of Biotechnology UG&MUG

Intercollegiate Faculty of Biotechnology UG&MUG

Last modified: 
2018, April 18 - 12:29pm

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Function, Evolution, Structure of Co-chaperones Having a J-domain 

International workshop of the Cell Stress Society International (CSSI)

4-7 April 2018, Gdansk, Poland


Baltic_Sea

 

Organizers:

Harm Kampinga (University Medical Center Groningen, The Netherlands) 

Elizabeth Craig (University of Wisconsin-Madison, USA)

Jaroslaw Marszalek (Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Poland)

Contact to organizer:

jpw_marszalek@biotech.ug.edu.pl

 

About workshop:

The workshop is focusing on the functional/structural diversity of J-domain proteins across cellular compartments and organisms. The workshop will not only provide a unique opportunity to gather together those with a central interest in J-protein function, but also will create a platform for discussing a new J-protein classification scheme.

The workshop is kindly supported by the Cell Stress Society International (ISSI), the Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of Gdansk, the University Medical Center Groningen.

The limited space at the workshop is full.

 

Program of the workshop:

Preliminary program of the workshop:

April 4th (Wednesday) – arrival day
7:00 pm Reception at the Novotel Gdańsk Marina Hotel
April 5th (Thursday)
9:00 - 10:40  Session I: introduction
Chair: Harm Kampinga

P1 Jaroslaw Marszalek, University of Gdansk, Poland
The idea behind the workshop, including overall organization and how discussion sessions will work; importance of evolutionary thinking for understanding J-proteins function and structure

P2 Jaime Huerta-Cepas, European Molecular Biology Laboratory, Heidelberg, Germany
Evolution of protein families, orthology versus paralogy, concept of orthologous groups, prediction of orthology based on the sequence and domain composition

P3 Matthias Mayer, Center for Molecular Biology of Heidelberg University, Germany
Molecular mechanism of J-domain stimulated ATPase activity of Hsp70s

P4 Alessandro Barducci, France Universite de Montpellier, CNRS, France
Combining molecular simulations and co-evolutionary analysis to investigate Hsp70/J-domain interactions

10:40 - 11:10 Coffee break
11:10 - 13:00 Session II: evolution and J-domain function
Chair: Nadinath Nillegoda

P5 Pierre Goloubinoff, University of Lausanne, Switzerland
J-domain co-chaperones as catalysts targeting misfolded or alternatively-folded polypeptides to the Hsp70 unfolding machineries

P6 Bartlomiej Tomiczek, University of Gdansk, Poland
Molecular mechanisms and evolution of J-domain/Hsp70 interaction

P7 Carlos Ramos, Institute of Chemistry, University of Campinas, Brazil,
Comparing the dynamics of isolated J-domain with those of full-length DnaJ proteins

P8 Pablo Pulido, University of Munich, Germany
DNAJ-related proteins in plants

P9 Chandan Sahi, Indian Institute of Science Education and Research Bhopal, India
Functional specificity and evolution of J proteins in eukaryotes

13:00 - 14:30 Lunch + break
14:30 - 16:00 Session III: substrate remodeling
Chair: Janine Kirstein

P10 Rina Rosenzweig, Weizmann Institute of Science, Israel
Substrate Remodeling by the Hsp70 System - What Can We Learn Using NMR

P11 Paolo De los Rios, Ecole Polytechnique Federale de Lausanne, Switzerland
A sequence-wise analysis of J-domain across the kingdoms of life

P12 Nadinath B. Nillegoda, Center for Molecular Biology of Heidelberg University, Germany
Inter/intraclass J-protein collaboration in Hsp70 chaperone-mediated protein quality control processes

P13 Krzysztof Liberek, University of Gdansk, Poland
Cytosolic J proteins from Saccharomyces cerevisiae in disaggregation and refolding of aggregated proteins

16:00 - 16:30 Coffee break
16:30 - 18:30 Discussion I: applying evolutionary thinking to J-protein research
Leaders: Jamie Huerta-Cepes, Jaroslaw Marszalek, Matthias Mayer
  • how tree thinking can help in recognizing patterns of sequence evolution and relatedness among J-proteins
  • how to apply the concept of an orthologous group to J-proteins
  • definition of a J-domain
  • how should we talk about proteins that have J-domains lacking HPD motif
  • how useful (or not) is the J-domain for predicting the relatedness among J-proteins
19:00 Dinner at the Novotel Gdansk Marina Hotel Restaurant
20:30 Beer and free time
April 6th (Friday)
9:00 - 10:30 Session IV: substrates specificity and functional diversification
Chair: Jason Gestwicki

P14 Harm Kampinga, University of Groningen, The Netherlands
Functional diversity of J-proteins

P15 Mikko Taipale, University of Toronto, Canada
Systematic proteomic profiling of Hsp40/Hsp70/client interactions

P16 Pierre Genevaux, Université Paul Sabatier CNRS, Toulouse, France
J- domain proteins in bacteria and their viruses

10:30 - 11:00 Coffee break
11:00 - 12:30 Session V: substrate triaging
Chair: Mikko Taipale

P17 Claes Andreasson, Stockholm University, Sweden
Hsp70 nucleotide exchange in the cytosol and nucleus

P18 Jason C. Young, McGill University, Montreal; Canada
Role of J-proteins in aggregation and folding

P19 Douglas Cyr, University of North Carolina, Chapel Hill, USA
Roles for J-Proteins in Membrane Protein Triage

P20 Linda M. Hendershot, St. Jude Children’s Research Hospital, Memphis, TN
Sequence recognition specificity of ER-localized DnaJ family members

13:00 - 14:30 Lunch + break
14:30 - 16:20 Session VI – Specialized Js
Chair: Maciej Zylicz

P21 Sabine Rospert, University of Freiburg, Germany
Two chaperones locked in an embrace: the ribosome-associated complex RAC

P22 Kazuhiro Nagata, Kyoto Sangyo University, Kyoto, Japan
Role of ER J-protein for the maintenance of ER homeostasis

P23 Richard Zimmermann, Saarland University, Homburg, Germany
The Hsp40/Hsp70 chaperone network of the human ER

P24 Ryo Ushioda, Kyoto Sangyo University, Kyoto, Japan
Maintenance of ER Homeostasis through Disulfide Reductase ERdj5

P25 Jason Gestwicki, University of California, San Francisco, USA
Assembly of Chaperone Complexes on Microtubule-Associated Protein Tau (MAPT)

16:20 - 16:40 Coffee break
16:40 - 18:40 Discussion II: domain organization outside the J-domain in relation to  function
Leaders: Linda M. Hendershot, Sabine Rospert, Jason Gestwicki
  • how J-proteins interact with 70s

o how to interpret HPD mutant data in experimental systems

o roles beyond HPD, e.g. G/F rich stretches (disease mutations!)

  • how do we think about 70-independent functions of proteins with a J-domain.
  • how we define and recognize domains (outside the J-domain) in J-proteins
  • functional diversity of domains: substrate-binding differences and overlap
  • Same J: different substrate fates: how?
  • collaboration between J-proteins;
  • could we improve current classification of J-proteins by using domain organization
19:30 - 22:00  Gala Dinner at the Restaurant "Otwarta"
April 7th (Saturday)
9:00 - 10:30 Session VII: J proteins and disease
Chair: Pierre Goloubinoff

P26 Janine KirsteinLeibniz-Institute for Molecular Pharmacology, Berlin, Germany
Analysis of mammalian and nematode J-proteins and their role in amyloid protein remodeling

P27 Maciej Zylicz, International Institute of Molecular and Cell Biology, Warsaw, Poland
Correlation between expression level of DNAJ family proteins and survival of cancer patients. Molecular mechanisms of DNAJ activity

P28 Cecilia Emanuelsson, Lund University, Sweden
Structural model of the oligomeric DNAJB6 showing dimers with an S/T-rich site for interaction with aggregation-prone peptides at the monomer-monomer interface

P29 Michael Cheetham, University College London, UK
The specialization of DNAJB2 to maintain neuronal proteostasis

10:30 - 11:00 Coffee break
11:00 - 12:30 Discussion III: targeting J-proteins to manipulate protein homeostasis networks in diseases
Leaders: Michael Cheetham, Douglas Cyr, Harm Kampinga
  • "fate decision" by J-proteins
  • How to target activities. Side effects?
  • J-protein networks 
12:30 - 13:00 Coffee break
13:00 - 13:30 Final Remarks
Leaders: Harm Kampinga, Jaroslaw Marszalek
  • specific questions and problems
  • prospects for future progress on classification and nomenclature of J-proteins
  • initiatives to continue these meetings?
  • plans for the meeting report paper for CSSI (to be published in CS&C)
  • plans for (major) consensus paper
13:30 - 14:30 Lunch
End of the meeting

 

List of participants:

Claes Andreasson
claes.andreasson@su.se
Stockholm University, Stockholm; Sweden

Alessandro Barducci
alessandro.barducci@cbs.cnrs.fr
Inserm, U1054, Montpellier, France

Michael Cheetham
michael.cheetham@ucl.ac.uk
University College London, UK

Douglas Cyr
douglas_cyr@med.unc.edu
University of North Carolina, Chapel Hill, USA

Rafal Dutkiewicz
rafal.dutkiewicz@biotech.ug.edu.pl
University of Gdansk, Gdansk, Poland

Cecilia Emanuelsson
Cecilia.Emanuelsson@biochemistry.lu.se
Lund University, Lund, Sweden

Pierre Genevaux
genevaux@ibcg.biotoul.fr 
Université de Toulouse, Toulouse, France

Jason Gestwicki
Jason.Gestwicki@ucsf.edu
University of California, San Francisco, USA

Pierre Goloubinoff
pierre.goloubinoff@unil.ch
University of Lausanne, Lausanne, Switzerland

Linda M. Hendershot
linda.hendershot@stjude.org
St. Jude Children’s Research Hospital, Memphis, USA

Jaime Huerta-Cepas
jaime.huerta@embl.de
European Molecular Biology Laboratory, Heidelberg, Germany

Harm H. Kampinga (organizer)
h.h.kampinga@med.umcg.nl
University of Groningen, Groningen, The Netherlands

Janine Kirstein
kirstein@fmp-berlin.de
Leibniz-Institute for Molecular Pharmacology (FMP), Berlin, Germany.

Krzysztof Liberek
krzysztof.liberek@biotech.ug.edu.pl
University of Gdansk, Gdansk, Poland

Matthias Mayer
m.mayer@zmbh.uni-heidelberg.de
Center for Molecular Biology of Heidelberg University (ZMBH), Heidelberg, Germany

Jaroslaw Marszalek (organizer)
jaroslaw.marszalek@biotech.ug.edu.pl
University of Gdansk, Gdansk, Poland

Kazuhiro Nagata
nagata@cc.kyoto-su.ac.jp
Kyoto Sangyo University, Kyoto, Japan

Nadinath B. Nillegoda
n.nillegoda@zmbh.uni-heidelberg.de
Center for Molecular Biology of Heidelberg University (ZMBH), Heidelberg, Germany

Pablo Pulido
pablo.pulido@lmu.de
Ludwig-Maximilians-University Munich, Planegg-Martinsried, Germany

Carlos Ramos
cramos@iqm.unicamp.br
University of Campinas, Campinas, Brazil

Paolo De los Rios
paolo.delosrios@epfl.ch
Ecole Polytechnique Federale de Lausanne (EPFL), Lausanne, Switzerland

Sabine Rospert
sabine.rospert@biochemie.uni-freiburg.de
Universitat Freiburg, Freiburg, Germany

Rina Rosenzweig
rina.rosenzweig@weizmann.ac.il
Weizmann Institute of Science, Israel 

Chandan Sahi
sahi@iiserb.ac.in
Indian Institute of Science Education and Research Bhopal, Bhauri Bhopal,
Madhya Pradesh, India

Mikko Taipale
mikko.taipale@utoronto.ca
University of Toronto, Canada

Bartlomiej Tomiczek
bartlomiej.tomiczek@ug.edu.pl
University of Gdansk, Gdansk, Poland

Ryo Ushioda
ryo3ussy3@cc.kyoto-su.ac.jp
Kyoto Sangyo University, Kyoto, Japan

Jason C. Young
jason.young2@mcgill.ca
McGill University, Montreal, Canada

Richard Zimmermann
richard.zimmermann@uks.eu
Saarland University, Homburg, Germany

Alicja Zylicz
alicja.zylicz@iimcb.gov.pl
International Institute of Molecular and Cell Biology, Warsaw, Poland

Maciej Zylicz
zylicz@fnp.org.pl
International Institute of Molecular and Cell Biology, Warsaw, Poland

 

Place of the workshop:

NOVOTEL GDANSK MARINA [link]

Jelitkowska 20

80-342 Gdansk, Poland

 

How to get to the Venue from Lech Walesa Airport?

Ride with Uber: https://www.uber.com/en-PL/

Take a taxi from the Airport to Jelitkowo, Novotel Marina Hotel. There is a taxi rank in front of the Airport. Cost is about 70 PLN (17 Euro).

 

Sponsors:

CSSI_logo     IFB_logo     UG_logo   UMCG_logo

 

Honourable patronage:

Rector of the University of Gdańsk, Professor Jerzy Piotr Gwizdała

Rector_UG

 

Gallery

All photos to download from Google Disc [link]

 

AttachmentSize
PDF icon JPW Program Final924.43 KB

Photos by Marcin Jeleń

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